LOCUS       sequence-121605-       12787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12787)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB106
FEATURES             Location/Qualifiers
     source          1..12787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2906..2908,2909..2911,2912..3622)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2951..2972)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3212..3231)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3559..3580
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      3647..3899
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3933..3949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3933..3949)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3946..3968)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3957..3973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3981..3987,3988..4005,4006..4011))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4026..4047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4132..4306
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4347..5372)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(5439..6114)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    6309..6333
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6854..7645
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7891..8479
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8380..8399
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8633..8650
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8665..8805
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8731..8750)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8891..8913
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9149..9343
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9409..10482)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10911..11540)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcatggt gagcaagggc gaggagctgt tcaccggggt
     2941 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3001 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3061 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt
     3121 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3181 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3241 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3301 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3361 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3421 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3481 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     3541 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3601 cggcatggac gagctgtaca agtgagctta gagctcgaat ttccccgatc gttcaaacat
     3661 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     3721 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     3781 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     3841 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     3901 ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3961 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4021 gcctaatgag tgagctaact cacattaggc tgaattaggc gcgcctattt ctgaattcaa
     4081 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     4141 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     4201 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     4261 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     4321 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
     4381 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     4441 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     4501 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     4561 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
     4621 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     4681 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     4741 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     4801 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     4861 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     4921 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     4981 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga
     5041 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag
     5101 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc
     5161 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     5221 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     5281 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg
     5341 acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccgga tctgcgaaag
     5401 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
     5461 aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc
     5521 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     5581 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     5641 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
     5701 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
     5761 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
     5821 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttcacatca atccacttgc
     5881 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     5941 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     6001 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     6061 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     6121 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     6181 accatgttgg caagctgctc tagccaatac gcaaaccgaa ttcagaaata aattcagcct
     6241 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     6301 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     6361 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     6421 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     6481 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     6541 aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc
     6601 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     6661 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     6721 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     6781 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     6841 aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     6901 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     6961 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     7021 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     7081 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     7141 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     7201 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     7261 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     7321 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     7381 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     7441 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     7501 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     7561 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     7621 gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct
     7681 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
     7741 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
     7801 gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7861 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7921 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7981 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     8041 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8101 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8161 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8221 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8281 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8341 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8401 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8461 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8521 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8581 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8641 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     8701 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     8761 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     8821 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     8881 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     8941 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     9001 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     9061 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     9121 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     9181 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     9241 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     9301 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     9361 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     9421 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     9481 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     9541 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     9601 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     9661 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     9721 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     9781 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     9841 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     9901 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     9961 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
    10021 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
    10081 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
    10141 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
    10201 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
    10261 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
    10321 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
    10381 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
    10441 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
    10501 ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg
    10561 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
    10621 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
    10681 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
    10741 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
    10801 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
    10861 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    10921 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    10981 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    11041 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    11101 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    11161 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    11221 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    11281 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    11341 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    11401 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    11461 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    11521 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    11581 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    11641 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    11701 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    11761 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    11821 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    11881 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    11941 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    12001 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    12061 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    12121 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    12181 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    12241 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    12301 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    12361 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    12421 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    12481 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    12541 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    12601 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    12661 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    12721 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    12781 taaacct
//
