LOCUS       sequence-121611-       12884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12884)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB307
FEATURES             Location/Qualifiers
     source          1..12884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2050..2052,2053..2055,2056..2766)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2095..2116)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2356..2375)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2703..2724
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      2791..3043
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3077..3093)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3077..3093)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3090..3112)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3101..3117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3125..3131,3132..3149,3150..3155))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3170..3191
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3276..3451
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(5535..6211)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(5578..5598)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6406..6430
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6951..7742
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7988..8576
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8477..8496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8730..8747
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8762..8902
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8828..8847)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8988..9010
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9246..9440
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9506..10579)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11008..11637)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2101 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2161 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2221 tggcccaccc tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac
     2281 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2341 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2401 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2461 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2521 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2581 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2641 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2701 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2761 tacaagtgag cttagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     2821 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     2881 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     2941 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     3001 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa
     3061 ccaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3121 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3181 aactcacatt aggctgaatt aggcgcgcct atttctgaat tcaacacatt gcggacgttt
     3241 ttaatgtact gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt
     3301 ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa
     3361 gggtttctta tatgctcaac acatgagcga aaccctatag gaaccctaaa ttcccttatc
     3421 tgggaactac tcacacatta ttatggagaa actcgaagtt agtatatagt ccttccatca
     3481 ccctctgtga taatctcctt aaaacttgct cccccaggaa tcataggcag aacgtgctcc
     3541 tggtggggaa ctatcacatc cagtaaataa ggaccgggag aatctagcat agtttgaatt
     3601 gctgctctta gttcatcctt cttcaccaca cgcgcatttg gcaccttgaa accgtctgca
     3661 actgtacaga agtcggggta gatctgctcc tcgtgatctg gatcacctaa gtaagtatgt
     3721 gctctgttgg ccttgtagaa tctatcctcc aattgtacga ccattcctag atgttggttg
     3781 tttaagatca tgatcttcac gggtaacttc tccacaaaaa ttgtagccaa ctcctgtacg
     3841 ttcatcacaa agctaccatc gccatcgatg tcgatcacag gaatatctgg ttgagttgcg
     3901 gcagccccca aagcagaagg aagtccgaat cccatggctc caagacctcc agatgtcaac
     3961 caacgccgcg gttcgttgta cttgaaccac tgggctgccc acatttgatg ttggccaaca
     4021 ccagtagata tgatggcatt tccaccagtg agttcgtaca gcgtctgtat ggcagattga
     4081 ggagcaatgg catcctcgaa tacgggaaac ttcattggcc atttctgctt ttgttcttct
     4141 agttctgcca cccagttgga gaagtcgaaa gtcctctttt gactcttcaa gagggcattg
     4201 atttcactta gcgcaggttt cacatcagca cacatggaca catggggctg tttgttcttt
     4261 ccaatttcag cagggtcaat atcgatatga acgatgctgg acctgctagc aaaagtctcc
     4321 agtttaccag tcactcgatc gtcgaaacgc actccaaatg ccaacaacag atctgcttgg
     4381 tccacggcgt agttggcgta aacagttcca tgcattccaa gcattttcaa acttaatgga
     4441 tcagactcgg gatacgctcc caaacccatt aaagtcgatg tgataggaat tccggtaagc
     4501 tcgacaaatt ccctcaactc ttcgctggcg tgattgcaac ctcctccaga atacactaca
     4561 ggcttcttag aactagaaat gagtcttata atctgctcga ttaacgttgg ttccggtggc
     4621 cgaggcagtc gttgtaagta gcccttcagt tgcattggct gagaccaatc tggaatagcc
     4681 atttgctgtt gcacgtcctt gggaatatct accaagactg ggcccggcct acctgaagct
     4741 gccaaaaaga aagcctccct gattacgcga gggatgtcat caatgttcat aacaaggtaa
     4801 ttgtgcttgg tgatcgatct cgtaacctcc acaattggag tttcttgaaa agcatcggta
     4861 ccaatcatcc tactggatac ttgacctgta atggccacta gtggcacact gtccaagagg
     4921 gcatcagcga gcccggtaac aagattggtc gctccaggac cggatgtggc tatacaaacc
     4981 cctactcgtc cagacgattt agcataacct tcagcagcga aaatctcccc ctgctcgtgg
     5041 cgacagagaa tgttgcggat gctgctggaa cgtgtaagag cctggtgaat ctccatagac
     5101 gctccgccgg gataagcgaa caccgttcta atcccttctc gctcaagagc ctccaccaaa
     5161 atgtcggctc ctttgcgggg ctcgtcatcc ttgtagcgag actgaaagtc ggacggggtt
     5221 ggtgcaacga gtgacttccc gctgctaagc cgtggtccac tcacatcgac tgcagactgg
     5281 atcttctcgc taacagcttg ggctttcact tccagtgaac gctcagtctt ccgagaggtg
     5341 ttgtatgcat gtcccctcag agcggctccc gagaagaaac cactcctcac ctttagctct
     5401 ttagctccct tgcaacaaga ccttgccgat ggctgcacct tctccaggct cgctatgctc
     5461 aaaggcacag ctgcgcatcc actaattgac gccatgctcg agagagatag atttgtagag
     5521 agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa
     5581 gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
     5641 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
     5701 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
     5761 ttatcgcaat tatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
     5821 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
     5881 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
     5941 gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     6001 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     6061 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     6121 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     6181 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     6241 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag
     6301 ccaatacgca aaccgaattc agaaataaat tcagcctaat tcggcgttaa ttcagtacat
     6361 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
     6421 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact
     6481 cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca
     6541 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
     6601 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
     6661 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     6721 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     6781 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     6841 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     6901 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
     6961 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     7021 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     7081 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     7141 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     7201 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     7261 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     7321 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     7381 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     7441 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     7501 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     7561 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     7621 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     7681 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     7741 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     7801 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     7861 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     7921 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7981 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8041 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8101 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     8161 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8221 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8281 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8341 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8401 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8461 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8521 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8581 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8641 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8701 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8761 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     8821 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     8881 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     8941 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     9001 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc
     9061 gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta
     9121 ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta
     9181 gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag
     9241 acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg
     9301 cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa
     9361 tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc
     9421 caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa
     9481 tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt
     9541 tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact
     9601 ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc
     9661 cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg
     9721 cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat
     9781 cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat
     9841 cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc
     9901 ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg
     9961 tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt
    10021 tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga
    10081 acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat
    10141 gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg
    10201 cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt
    10261 cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca
    10321 cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa
    10381 tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg
    10441 cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg
    10501 cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg
    10561 atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg
    10621 ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca
    10681 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa
    10741 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag
    10801 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat
    10861 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca
    10921 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt
    10981 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag
    11041 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc
    11101 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc
    11161 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga
    11221 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc
    11281 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa
    11341 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac
    11401 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc
    11461 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg
    11521 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt
    11581 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg
    11641 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc
    11701 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg
    11761 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc
    11821 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc
    11881 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg
    11941 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt
    12001 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa
    12061 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc
    12121 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc
    12181 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga
    12241 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg
    12301 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct
    12361 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg
    12421 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt
    12481 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt
    12541 cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc
    12601 cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact
    12661 ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc
    12721 gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga
    12781 agccgactgc actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac
    12841 cctgtggttg gcatgcacat acaaatggac gaacggataa acct
//
