LOCUS       sequence-121615-       14677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14677)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 14677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB308
FEATURES             Location/Qualifiers
     source          1..14677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2302..2961
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2368..2387)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3487..3506
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(3805..3807,3808..3810,3811..4521)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3850..3871)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4111..4130)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4458..4479
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4584..4836
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4870..4886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4870..4886)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4883..4905)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4894..4910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4918..4924,4925..4942,4943..4948))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4963..4984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5069..5244
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(7328..8004)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(7371..7391)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    8199..8223
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8744..9535
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9781..10369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10270..10289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10523..10540
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10555..10695
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10621..10640)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10781..10803
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11039..11233
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11299..12372)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12801..13430)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcgctggag gctccggtgg gagcatggtg agcaagggcg aggagctgtt caccggggtg
     3841 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3901 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3961 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gatgtgcttc
     4021 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4081 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4141 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4201 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     4261 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     4321 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4381 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     4441 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4501 ggcatggacg agctgtacaa gtaagcttgc atgcctgcag gtcgactcta gaggatcccc
     4561 gggtaccgag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     4621 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     4681 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     4741 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     4801 aattatcgcg cgcggtgtca tctatgttac tagatcggga attggttccg gaaccaattc
     4861 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4921 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4981 attaggctga attaggcgcg cctatttctg aattcaacac attgcggacg tttttaatgt
     5041 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
     5101 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
     5161 ttatatgctc aacacatgag cgaaacccta taggaaccct aaattccctt atctgggaac
     5221 tactcacaca ttattatgga gaaactcgaa gttagtatat agtccttcca tcaccctctg
     5281 tgataatctc cttaaaactt gctcccccag gaatcatagg cagaacgtgc tcctggtggg
     5341 gaactatcac atccagtaaa taaggaccgg gagaatctag catagtttga attgctgctc
     5401 ttagttcatc cttcttcacc acacgcgcat ttggcacctt gaaaccgtct gcaactgtac
     5461 agaagtcggg gtagatctgc tcctcgtgat ctggatcacc taagtaagta tgtgctctgt
     5521 tggccttgta gaatctatcc tccaattgta cgaccattcc tagatgttgg ttgtttaaga
     5581 tcatgatctt cacgggtaac ttctccacaa aaattgtagc caactcctgt acgttcatca
     5641 caaagctacc atcgccatcg atgtcgatca caggaatatc tggttgagtt gcggcagccc
     5701 ccaaagcaga aggaagtccg aatcccatgg ctccaagacc tccagatgtc aaccaacgcc
     5761 gcggttcgtt gtacttgaac cactgggctg cccacatttg atgttggcca acaccagtag
     5821 atatgatggc atttccacca gtgagttcgt acagcgtctg tatggcagat tgaggagcaa
     5881 tggcatcctc gaatacggga aacttcattg gccatttctg cttttgttct tctagttctg
     5941 ccacccagtt ggagaagtcg aaagtcctct tttgactctt caagagggca ttgatttcac
     6001 ttagcgcagg tttcacatca gcacacatgg acacatgggg ctgtttgttc tttccaattt
     6061 cagcagggtc aatatcgata tgaacgatgc tggacctgct agcaaaagtc tccagtttac
     6121 cagtcactcg atcgtcgaaa cgcactccaa atgccaacaa cagatctgct tggtccacgg
     6181 cgtagttggc gtaaacagtt ccatgcattc caagcatttt caaacttaat ggatcagact
     6241 cgggatacgc tcccaaaccc attaaagtcg atgtgatagg aattccggta agctcgacaa
     6301 attccctcaa ctcttcgctg gcgtgattgc aacctcctcc agaatacact acaggcttct
     6361 tagaactaga aatgagtctt ataatctgct cgattaacgt tggttccggt ggccgaggca
     6421 gtcgttgtaa gtagcccttc agttgcattg gctgagacca atctggaata gccatttgct
     6481 gttgcacgtc cttgggaata tctaccaaga ctgggcccgg cctacctgaa gctgccaaaa
     6541 agaaagcctc cctgattacg cgagggatgt catcaatgtt cataacaagg taattgtgct
     6601 tggtgatcga tctcgtaacc tccacaattg gagtttcttg aaaagcatcg gtaccaatca
     6661 tcctactgga tacttgacct gtaatggcca ctagtggcac actgtccaag agggcatcag
     6721 cgagcccggt aacaagattg gtcgctccag gaccggatgt ggctatacaa acccctactc
     6781 gtccagacga tttagcataa ccttcagcag cgaaaatctc cccctgctcg tggcgacaga
     6841 gaatgttgcg gatgctgctg gaacgtgtaa gagcctggtg aatctccata gacgctccgc
     6901 cgggataagc gaacaccgtt ctaatccctt ctcgctcaag agcctccacc aaaatgtcgg
     6961 ctcctttgcg gggctcgtca tccttgtagc gagactgaaa gtcggacggg gttggtgcaa
     7021 cgagtgactt cccgctgcta agccgtggtc cactcacatc gactgcagac tggatcttct
     7081 cgctaacagc ttgggctttc acttccagtg aacgctcagt cttccgagag gtgttgtatg
     7141 catgtcccct cagagcggct cccgagaaga aaccactcct cacctttagc tctttagctc
     7201 ccttgcaaca agaccttgcc gatggctgca ccttctccag gctcgctatg ctcaaaggca
     7261 cagctgcgca tccactaatt gacgccatgc tcgagagaga tagatttgta gagagagact
     7321 ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
     7381 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca
     7441 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
     7501 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
     7561 aattatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
     7621 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
     7681 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
     7741 gctccaccat gttcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     7801 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     7861 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
     7921 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
     7981 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
     8041 ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac
     8101 gcaaaccgaa ttcagaaata aattcagcct aattcggcgt taattcagta cattaaaaac
     8161 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
     8221 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
     8281 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
     8341 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
     8401 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
     8461 aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     8521 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     8581 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     8641 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     8701 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat
     8761 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     8821 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     8881 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     8941 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     9001 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     9061 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     9121 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     9181 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     9241 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     9301 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     9361 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     9421 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     9481 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     9541 agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
     9601 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
     9661 taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc
     9721 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9781 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9841 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9901 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     9961 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10021 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10081 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    10141 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    10201 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    10261 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    10321 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    10381 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    10441 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    10501 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
    10561 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
    10621 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
    10681 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
    10741 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
    10801 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
    10861 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
    10921 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
    10981 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
    11041 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
    11101 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
    11161 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
    11221 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
    11281 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
    11341 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
    11401 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
    11461 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
    11521 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
    11581 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
    11641 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
    11701 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
    11761 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
    11821 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
    11881 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
    11941 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
    12001 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
    12061 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
    12121 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
    12181 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
    12241 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
    12301 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
    12361 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
    12421 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
    12481 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
    12541 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
    12601 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
    12661 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
    12721 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
    12781 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
    12841 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
    12901 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
    12961 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
    13021 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
    13081 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
    13141 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
    13201 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
    13261 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
    13321 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
    13381 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
    13441 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
    13501 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
    13561 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
    13621 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
    13681 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
    13741 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
    13801 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
    13861 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
    13921 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
    13981 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
    14041 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
    14101 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
    14161 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
    14221 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
    14281 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
    14341 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
    14401 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
    14461 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
    14521 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
    14581 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
    14641 ttggcatgca catacaaatg gacgaacgga taaacct
//
