LOCUS       sequence-121620-       11059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB409
FEATURES             Location/Qualifiers
     source          1..11059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2050..2115
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      2134..2386
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2420..2436)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2420..2436)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2433..2455)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2444..2460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2468..2474,2475..2492,2493..2498))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2513..2534
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    2619..2793
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2850..3647)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2961..2980)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3571..3590
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3710..4386)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(3753..3773)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    4581..4605
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5126..5917
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6163..6751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6652..6671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6905..6922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6937..7077
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7003..7022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7163..7185
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7421..7615
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7681..8754)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9183..9812)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagcg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     2101 gatgacgatg acaagtagag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     2161 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     2221 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     2281 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     2341 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attggttccg
     2401 gaaccaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2461 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2521 gctaactcac attaggctga attaggcgcg cctatttctg aattcaacac attgcggacg
     2581 tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt agtactggat
     2641 tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac
     2701 taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta
     2761 tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg actctagcta
     2821 gaggatcgat ccgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     2881 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     2941 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     3001 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     3061 ttcggcaagc aggcatcgcc atgtgtcacg acgagatcct cgccgtcggg catgcgcgcc
     3121 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     3181 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     3241 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     3301 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     3361 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     3421 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctggagttc attcagggca
     3481 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     3541 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     3601 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tccccatggt cgatcgacag
     3661 atctgcgaaa gctcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc
     3721 tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt
     3781 gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg
     3841 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
     3901 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg
     3961 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc
     4021 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg
     4081 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttcacat
     4141 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
     4201 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
     4261 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
     4321 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
     4381 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
     4441 gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg aattcagaaa
     4501 taaattcagc ctaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     4561 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     4621 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     4681 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     4741 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     4801 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
     4861 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     4921 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     4981 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     5041 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     5101 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
     5161 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     5221 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     5281 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     5341 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     5401 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     5461 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     5521 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     5581 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     5641 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     5701 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     5761 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     5821 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     5881 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
     5941 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     6001 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     6061 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     6121 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6181 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6241 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6301 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6361 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6421 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6481 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6541 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6601 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6661 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6721 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6781 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6841 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6901 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     6961 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     7021 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     7081 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     7141 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     7201 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     7261 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     7321 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     7381 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     7441 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     7501 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
     7561 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
     7621 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
     7681 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
     7741 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
     7801 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
     7861 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
     7921 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
     7981 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
     8041 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
     8101 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
     8161 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
     8221 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
     8281 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
     8341 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
     8401 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
     8461 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
     8521 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
     8581 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
     8641 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
     8701 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat
     8761 tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg
     8821 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt
     8881 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt
     8941 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc
     9001 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac
     9061 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc
     9121 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta
     9181 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg
     9241 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg
     9301 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc
     9361 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt
     9421 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg
     9481 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca
     9541 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc
     9601 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc
     9661 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg
     9721 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg
     9781 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta
     9841 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc
     9901 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca
     9961 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg
    10021 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa
    10081 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc
    10141 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct
    10201 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg
    10261 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg
    10321 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc
    10381 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc
    10441 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg
    10501 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag
    10561 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg
    10621 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc
    10681 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg
    10741 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc
    10801 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag
    10861 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat
    10921 gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag
    10981 gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa
    11041 tggacgaacg gataaacct
//
