LOCUS       sequence-121624-       12793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB410
FEATURES             Location/Qualifiers
     source          1..12793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2302..2961
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2368..2387)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3487..3506
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3784..3849
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3868..4120
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4154..4170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4154..4170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4167..4189)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4178..4194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4202..4208,4209..4226,4227..4232))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4247..4268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4353..4527
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4584..5381)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4695..4714)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5305..5324
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5444..6120)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(5487..5507)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6315..6339
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6860..7651
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7897..8485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8386..8405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8639..8656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8671..8811
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8737..8756)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8897..8919
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9155..9349
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9415..10488)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10917..11546)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcgactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     3841 gatgacaagt agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
     3901 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     3961 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     4021 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     4081 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattggt tccggaacca
     4141 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4201 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4261 tcacattagg ctgaattagg cgcgcctatt tctgaattca acacattgcg gacgttttta
     4321 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
     4381 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     4441 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg
     4501 aactactcac acattattat ggagaaactc gagcttgtcg atcgactcta gctagaggat
     4561 cgatccgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     4621 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     4681 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     4741 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     4801 aagcaggcat cgccatgtgt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     4861 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     4921 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     4981 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     5041 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     5101 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     5161 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgga gttcattcag ggcaccggac
     5221 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     5281 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     5341 gccggagaac ctgcgtgcaa tccatcttgt tcaatcccca tggtcgatcg acagatctgc
     5401 gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca
     5461 aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt
     5521 catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa
     5581 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     5641 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
     5701 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
     5761 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
     5821 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttc acatcaatcc
     5881 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
     5941 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
     6001 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
     6061 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
     6121 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
     6181 tgctccacca tgttggcaag ctgctctagc caatacgcaa accgaattca gaaataaatt
     6241 cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     6301 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     6361 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     6421 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     6481 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     6541 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     6601 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     6661 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     6721 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     6781 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     6841 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     6901 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     6961 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     7021 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     7081 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     7141 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     7201 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     7261 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     7321 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     7381 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     7441 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     7501 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     7561 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     7621 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     7681 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     7741 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     7801 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8041 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8641 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     8701 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     8761 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     8821 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     8881 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     8941 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     9001 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     9061 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     9121 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     9181 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     9241 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     9301 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     9361 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     9421 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     9481 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     9541 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     9601 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     9661 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     9721 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     9781 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     9841 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     9901 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     9961 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
    10021 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
    10081 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
    10141 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
    10201 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
    10261 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
    10321 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
    10381 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
    10441 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
    10501 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
    10561 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
    10621 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
    10681 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
    10741 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
    10801 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
    10861 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
    10921 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
    10981 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
    11041 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
    11101 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
    11161 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
    11221 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
    11281 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    11341 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    11401 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    11461 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    11521 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    11581 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    11641 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    11701 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    11761 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    11821 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    11881 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    11941 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    12001 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    12061 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    12121 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    12181 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    12241 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    12301 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    12361 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    12421 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    12481 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    12541 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    12601 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    12661 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    12721 gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
    12781 aacggataaa cct
//
