LOCUS       sequence-121625-       12130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12130)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB111
FEATURES             Location/Qualifiers
     source          1..12130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2906..2971
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      2990..3242
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3276..3292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3276..3292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3289..3311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3300..3316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3324..3330,3331..3348,3349..3354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3369..3390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3475..3649
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3690..4715)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(4782..5457)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    5652..5676
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6197..6988
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7234..7822
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7723..7742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7976..7993
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8008..8148
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8074..8093)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8234..8256
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8492..8686
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8752..9825)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10254..10883)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgacta caaagaccat gacggtgatt ataaagatca
     2941 tgacatcgac tacaaggatg acgatgacaa gtagagctcg aatttccccg atcgttcaaa
     3001 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     3061 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     3121 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     3181 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     3241 tcgggaattg gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     3301 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3361 ggtgcctaat gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt
     3421 caacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg
     3481 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
     3541 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg
     3601 aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt
     3661 cgatcgacag atcccggtcg gcatctactc tatttctttg ccctcggacg agtgctgggg
     3721 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
     3781 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
     3841 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
     3901 tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg atcctgcaag ctccggatgc
     3961 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
     4021 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
     4081 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
     4141 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
     4201 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
     4261 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
     4321 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tagcctccgc gaccggttgt
     4381 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgaacggcgg
     4441 gagatgcaat aggtcaggct ctcgctaaac tccccaatgt caagcacttc cggaatcggg
     4501 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
     4561 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
     4621 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
     4681 tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccc ggatctgcga
     4741 aagctcgaga gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa
     4801 tgaaatgaac ttccttatat agaggaaggt cttgcgaagg atagtgggat tgtgcgtcat
     4861 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
     4921 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
     4981 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
     5041 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
     5101 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
     5161 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttcaca tcaatccact
     5221 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
     5281 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
     5341 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
     5401 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
     5461 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
     5521 tccaccatgt tggcaagctg ctctagccaa tacgcaaacc gaattcagaa ataaattcag
     5581 cctaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     5641 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     5701 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     5761 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     5821 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     5881 tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata
     5941 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     6001 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     6061 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     6121 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     6181 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     6241 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     6301 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     6361 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     6421 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     6481 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     6541 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     6601 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     6661 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     6721 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     6781 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     6841 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     6901 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     6961 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
     7021 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
     7081 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
     7141 attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7381 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7621 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7861 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7921 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7981 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     8041 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8101 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     8161 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     8221 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     8281 gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc
     8341 gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat
     8401 aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg
     8461 cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt
     8521 tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt
     8581 cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg
     8641 gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt
     8701 tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc
     8761 ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg
     8821 ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac
     8881 ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa
     8941 caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc
     9001 gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc
     9061 ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc
     9121 ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac
     9181 caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg
     9241 aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt
     9301 gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg
     9361 gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca
     9421 cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta
     9481 gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat
     9541 gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg
     9601 ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga
     9661 ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc
     9721 gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag
     9781 cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc
     9841 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     9901 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     9961 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
    10021 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
    10081 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
    10141 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
    10201 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
    10261 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
    10321 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
    10381 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
    10441 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
    10501 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
    10561 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
    10621 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
    10681 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
    10741 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
    10801 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
    10861 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
    10921 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
    10981 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
    11041 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
    11101 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
    11161 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
    11221 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
    11281 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
    11341 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
    11401 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
    11461 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
    11521 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
    11581 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
    11641 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
    11701 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
    11761 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
    11821 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
    11881 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
    11941 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
    12001 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
    12061 caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
    12121 ggataaacct
//
