LOCUS       sequence-121628-       11915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 11915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB411
FEATURES             Location/Qualifiers
     source          1..11915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2906..2971
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      2990..3242
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3276..3292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3276..3292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3289..3311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3300..3316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3324..3330,3331..3348,3349..3354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3369..3390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3475..3649
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3706..4503)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3817..3836)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4427..4446
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4566..5242)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    5437..5461
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5982..6773
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7019..7607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7508..7527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7761..7778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7793..7933
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7859..7878)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8019..8041
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8277..8471
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8537..9610)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10039..10668)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgacta caaagaccat gacggtgatt ataaagatca
     2941 tgacatcgac tacaaggatg acgatgacaa gtagagctcg aatttccccg atcgttcaaa
     3001 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     3061 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     3121 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     3181 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     3241 tcgggaattg gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     3301 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3361 ggtgcctaat gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt
     3421 caacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg
     3481 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
     3541 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg
     3601 aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt
     3661 cgatcgactc tagctagagg atcgatccga accccagagt cccgctcaga agaactcgtc
     3721 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     3781 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     3841 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     3901 attttccacc atgatattcg gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc
     3961 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     4021 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     4081 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     4141 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     4201 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     4261 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
     4321 gagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     4381 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     4441 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatccc
     4501 catggtcgat cgacagatct gcgaaagctc gagagagata gatttgtaga gagagactgg
     4561 tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc
     4621 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
     4681 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
     4741 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
     4801 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
     4861 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
     4921 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
     4981 tccaccatgt tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
     5041 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
     5101 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
     5161 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
     5221 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
     5281 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
     5341 aaaccgaatt cagaaataaa ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt
     5401 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     5461 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     5521 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     5581 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     5641 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa
     5701 ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     5761 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     5821 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     5881 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     5941 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg
     6001 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     6061 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     6121 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     6181 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     6241 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     6301 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     6361 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     6421 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     6481 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     6541 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     6601 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     6661 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     6721 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag
     6781 ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc
     6841 actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta
     6901 agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc aaaatccctt
     6961 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7021 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7081 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7141 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     7201 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7261 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7321 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7381 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7441 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7501 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7561 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7621 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7681 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7741 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     7801 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     7861 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     7921 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     7981 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     8041 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
     8101 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
     8161 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
     8221 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
     8281 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
     8341 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
     8401 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
     8461 tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta
     8521 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
     8581 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
     8641 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
     8701 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
     8761 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
     8821 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
     8881 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
     8941 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
     9001 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
     9061 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
     9121 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
     9181 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
     9241 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
     9301 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
     9361 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
     9421 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
     9481 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
     9541 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
     9601 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
     9661 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
     9721 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
     9781 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
     9841 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
     9901 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
     9961 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
    10021 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
    10081 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
    10141 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
    10201 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
    10261 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
    10321 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
    10381 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
    10441 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
    10501 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
    10561 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
    10621 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
    10681 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
    10741 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
    10801 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
    10861 gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc
    10921 tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct
    10981 cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga
    11041 aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt
    11101 acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct
    11161 ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa
    11221 acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg
    11281 aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg
    11341 tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct
    11401 cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca
    11461 caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg
    11521 tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt
    11581 ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg
    11641 cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac
    11701 taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt
    11761 aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
    11821 cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt
    11881 ggcatgcaca tacaaatgga cgaacggata aacct
//
