LOCUS       sequence-121632-       11866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11866)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 11866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB412
FEATURES             Location/Qualifiers
     source          1..11866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2941..3193
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3227..3243)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3227..3243)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3240..3262)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3251..3267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3275..3281,3282..3299,3300..3305))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3320..3341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3426..3600
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3657..4454)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3768..3787)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4378..4397
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4517..5193)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4560..4580)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5388..5412
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5933..6724
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6970..7558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7459..7478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7712..7729
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7744..7884
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7810..7829)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7970..7992
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8228..8422
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8488..9561)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9990..10619)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagca tggctagtga agctcgaaaa acaaagaaaa aaatcaaagg gattcagcaa
     2101 gccactgcag gagtctcaca agacacttcg gaaaatccta acaaaacaat agttcctgca
     2161 gcattaccac agctcacccc taccttggtg tcactgctgg aggtgattga acccgaggtg
     2221 ttgtatgcag gatatgatag ctctgttcca gattcagcat ggagaattat gaccacactc
     2281 aacatgttag gtgggcgtca agtgattgca gcagtgaaat gggcaaaggc gataccaggc
     2341 ttcagaaact tacacctgga tgaccaaatg accctgctac agtactcatg gatgtttctc
     2401 atggcatttg ccctgggttg gagatcatac agacaatcaa gtggaaacct gctctgcttt
     2461 gctcctgatc tgattattaa tgagcagaga atgtctctac cctgcatgta tgaccaatgt
     2521 aaacacatgc tgtttgtctc ctctgaatta caaagattgc aggtatccta tgaagagtat
     2581 ctctgtatga aaaccttact gcttctctcc tcagttccta aggaaggtct gaagagccaa
     2641 gagttatttg atgagattcg aatgacttat atcaaagagc taggaaaagc catcgtcaaa
     2701 agggaaggga actccagtca gaactggcaa cggttttacc aactgacaaa acttctggac
     2761 tccatgcatg aggtggttga gaatctcctt acctactgct tccagacatt tttggataag
     2821 accatgagta ttgaattccc agagatgtta gctgaaatca tcactaatca gataccaaaa
     2881 tattcaaatg gaaatatcaa gaagctgctg tttcatcaaa aatagagctc gaatttcccc
     2941 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     3001 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     3061 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     3121 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     3181 atgttactag atcgggaatt ggttccggaa ccaattcgta atcatggtca tagctgtttc
     3241 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3301 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aggctgaatt aggcgcgcct
     3361 atttctgaat tcaacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
     3421 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
     3481 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
     3541 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
     3601 ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag tcccgctcag
     3661 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     3721 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     3781 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     3841 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg tgtcacgacg
     3901 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     3961 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     4021 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     4081 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     4141 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     4201 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     4261 gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     4321 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     4381 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     4441 tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat agatttgtag
     4501 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
     4561 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     4621 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     4681 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     4741 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     4801 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     4861 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     4921 gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg
     4981 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     5041 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
     5101 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
     5161 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
     5221 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct
     5281 agccaatacg caaaccgaat tcagaaataa attcagccta attcggcgtt aattcagtac
     5341 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     5401 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     5461 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     5521 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     5581 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     5641 cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     5701 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     5761 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     5821 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     5881 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga
     5941 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     6001 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     6061 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     6121 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     6181 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     6241 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     6301 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     6361 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     6421 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     6481 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     6541 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     6601 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     6661 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     6721 ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     6781 gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt
     6841 tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac
     6901 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6961 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7021 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7081 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7141 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7201 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7261 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7321 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7381 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7441 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7501 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7561 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7621 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7681 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7741 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     7801 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     7861 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     7921 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     7981 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
     8041 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
     8101 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
     8161 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
     8221 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
     8281 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
     8341 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
     8401 cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc
     8461 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
     8521 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
     8581 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
     8641 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
     8701 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
     8761 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
     8821 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
     8881 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
     8941 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
     9001 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
     9061 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
     9121 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
     9181 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
     9241 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
     9301 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
     9361 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
     9421 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
     9481 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
     9541 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
     9601 ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
     9661 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
     9721 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
     9781 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
     9841 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
     9901 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
     9961 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
    10021 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
    10081 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
    10141 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
    10201 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
    10261 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
    10321 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
    10381 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
    10441 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
    10501 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
    10561 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
    10621 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
    10681 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
    10741 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
    10801 gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct
    10861 ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca
    10921 tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc
    10981 gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc
    11041 aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt
    11101 gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg
    11161 gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc
    11221 gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc
    11281 ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc
    11341 ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct
    11401 ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg
    11461 gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag
    11521 ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag
    11581 gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta
    11641 ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg
    11701 tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca
    11761 gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga
    11821 accctgtggt tggcatgcac atacaaatgg acgaacggat aaacct
//
