LOCUS       sequence-121635-       14768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14768)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 14768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB313
FEATURES             Location/Qualifiers
     source          1..14768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2302..2961
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2368..2387)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3487..3506
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4675..4927
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4961..4977)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4961..4977)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4974..4996)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4985..5001
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5009..5015,5016..5033,5034..5039))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5054..5075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5160..5335
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(7419..8095)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(7462..7482)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    8290..8314
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8835..9626
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9872..10460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10361..10380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10614..10631
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10646..10786
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10712..10731)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10872..10894
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11130..11324
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11390..12463)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12892..13521)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcatggcta gtgaagctcg aaaaacaaag aaaaaaatca aagggattca gcaagccact
     3841 gcaggagtct cacaagacac ttcggaaaat cctaacaaaa caatagttcc tgcagcatta
     3901 ccacagctca cccctacctt ggtgtcactg ctggaggtga ttgaacccga ggtgttgtat
     3961 gcaggatatg atagctctgt tccagattca gcatggagaa ttatgaccac actcaacatg
     4021 ttaggtgggc gtcaagtgat tgcagcagtg aaatgggcaa aggcgatacc aggcttcaga
     4081 aacttacacc tggatgacca aatgaccctg ctacagtact catggatgtt tctcatggca
     4141 tttgccctgg gttggagatc atacagacaa tcaagtggaa acctgctctg ctttgctcct
     4201 gatctgatta ttaatgagca gagaatgtct ctaccctgca tgtatgacca atgtaaacac
     4261 atgctgtttg tctcctctga attacaaaga ttgcaggtat cctatgaaga gtatctctgt
     4321 atgaaaacct tactgcttct ctcctcagtt cctaaggaag gtctgaagag ccaagagtta
     4381 tttgatgaga ttcgaatgac ttatatcaaa gagctaggaa aagccatcgt caaaagggaa
     4441 gggaactcca gtcagaactg gcaacggttt taccaactga caaaacttct ggactccatg
     4501 catgaggtgg ttgagaatct ccttacctac tgcttccaga catttttgga taagaccatg
     4561 agtattgaat tcccagagat gttagctgaa atcatcacta atcagatacc aaaatattca
     4621 aatggaaata tcaagaagct gctgtttcat caaaaataga gctcgaattt ccccgatcgt
     4681 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     4741 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     4801 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     4861 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     4921 ctagatcggg aattggttcc ggaaccaatt cgtaatcatg gtcatagctg tttcctgtgt
     4981 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5041 cctggggtgc ctaatgagtg agctaactca cattaggctg aattaggcgc gcctatttct
     5101 gaattcaaca cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg
     5161 atctggattt tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat
     5221 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
     5281 ataggaaccc taaattccct tatctgggaa ctactcacac attattatgg agaaactcga
     5341 agttagtata tagtccttcc atcaccctct gtgataatct ccttaaaact tgctccccca
     5401 ggaatcatag gcagaacgtg ctcctggtgg ggaactatca catccagtaa ataaggaccg
     5461 ggagaatcta gcatagtttg aattgctgct cttagttcat ccttcttcac cacacgcgca
     5521 tttggcacct tgaaaccgtc tgcaactgta cagaagtcgg ggtagatctg ctcctcgtga
     5581 tctggatcac ctaagtaagt atgtgctctg ttggccttgt agaatctatc ctccaattgt
     5641 acgaccattc ctagatgttg gttgtttaag atcatgatct tcacgggtaa cttctccaca
     5701 aaaattgtag ccaactcctg tacgttcatc acaaagctac catcgccatc gatgtcgatc
     5761 acaggaatat ctggttgagt tgcggcagcc cccaaagcag aaggaagtcc gaatcccatg
     5821 gctccaagac ctccagatgt caaccaacgc cgcggttcgt tgtacttgaa ccactgggct
     5881 gcccacattt gatgttggcc aacaccagta gatatgatgg catttccacc agtgagttcg
     5941 tacagcgtct gtatggcaga ttgaggagca atggcatcct cgaatacggg aaacttcatt
     6001 ggccatttct gcttttgttc ttctagttct gccacccagt tggagaagtc gaaagtcctc
     6061 ttttgactct tcaagagggc attgatttca cttagcgcag gtttcacatc agcacacatg
     6121 gacacatggg gctgtttgtt ctttccaatt tcagcagggt caatatcgat atgaacgatg
     6181 ctggacctgc tagcaaaagt ctccagttta ccagtcactc gatcgtcgaa acgcactcca
     6241 aatgccaaca acagatctgc ttggtccacg gcgtagttgg cgtaaacagt tccatgcatt
     6301 ccaagcattt tcaaacttaa tggatcagac tcgggatacg ctcccaaacc cattaaagtc
     6361 gatgtgatag gaattccggt aagctcgaca aattccctca actcttcgct ggcgtgattg
     6421 caacctcctc cagaatacac tacaggcttc ttagaactag aaatgagtct tataatctgc
     6481 tcgattaacg ttggttccgg tggccgaggc agtcgttgta agtagccctt cagttgcatt
     6541 ggctgagacc aatctggaat agccatttgc tgttgcacgt ccttgggaat atctaccaag
     6601 actgggcccg gcctacctga agctgccaaa aagaaagcct ccctgattac gcgagggatg
     6661 tcatcaatgt tcataacaag gtaattgtgc ttggtgatcg atctcgtaac ctccacaatt
     6721 ggagtttctt gaaaagcatc ggtaccaatc atcctactgg atacttgacc tgtaatggcc
     6781 actagtggca cactgtccaa gagggcatca gcgagcccgg taacaagatt ggtcgctcca
     6841 ggaccggatg tggctataca aacccctact cgtccagacg atttagcata accttcagca
     6901 gcgaaaatct ccccctgctc gtggcgacag agaatgttgc ggatgctgct ggaacgtgta
     6961 agagcctggt gaatctccat agacgctccg ccgggataag cgaacaccgt tctaatccct
     7021 tctcgctcaa gagcctccac caaaatgtcg gctcctttgc ggggctcgtc atccttgtag
     7081 cgagactgaa agtcggacgg ggttggtgca acgagtgact tcccgctgct aagccgtggt
     7141 ccactcacat cgactgcaga ctggatcttc tcgctaacag cttgggcttt cacttccagt
     7201 gaacgctcag tcttccgaga ggtgttgtat gcatgtcccc tcagagcggc tcccgagaag
     7261 aaaccactcc tcacctttag ctctttagct cccttgcaac aagaccttgc cgatggctgc
     7321 accttctcca ggctcgctat gctcaaaggc acagctgcgc atccactaat tgacgccatg
     7381 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
     7441 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
     7501 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
     7561 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
     7621 gcatcttgaa cgatagcctt tcctttatcg caattatggc atttgtaggt gccaccttcc
     7681 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
     7741 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
     7801 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc aatccacttg
     7861 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     7921 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     7981 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     8041 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     8101 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     8161 caccatgttg gcaagctgct ctagccaata cgcaaaccga attcagaaat aaattcagcc
     8221 taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
     8281 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
     8341 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
     8401 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
     8461 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
     8521 taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag
     8581 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     8641 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     8701 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     8761 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     8821 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     8881 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     8941 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     9001 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     9061 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     9121 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     9181 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     9241 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     9301 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     9361 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     9421 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     9481 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     9541 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     9601 agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc
     9661 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     9721 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     9781 tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9841 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9901 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9961 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
    10021 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    10081 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    10141 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10201 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10261 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10321 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10381 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10441 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10501 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    10561 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    10621 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
    10681 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    10741 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
    10801 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
    10861 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
    10921 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
    10981 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
    11041 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
    11101 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
    11161 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
    11221 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
    11281 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
    11341 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
    11401 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
    11461 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
    11521 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
    11581 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
    11641 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
    11701 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
    11761 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
    11821 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
    11881 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
    11941 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
    12001 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
    12061 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
    12121 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
    12181 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
    12241 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
    12301 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
    12361 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
    12421 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt
    12481 gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc
    12541 gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc
    12601 catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact
    12661 ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg
    12721 cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg
    12781 ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc
    12841 cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag
    12901 ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc
    12961 tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat
    13021 acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat
    13081 gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac
    13141 ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct
    13201 tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc
    13261 cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg
    13321 gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc
    13381 ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag
    13441 ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt
    13501 aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc
    13561 atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga
    13621 cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca
    13681 gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac
    13741 acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc
    13801 tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca
    13861 gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg
    13921 gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca
    13981 gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga
    14041 tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct
    14101 cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga
    14161 acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc
    14221 tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc
    14281 tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa
    14341 gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct
    14401 tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc
    14461 tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc
    14521 ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg
    14581 aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag
    14641 aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca
    14701 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
    14761 ataaacct
//
