LOCUS       sequence-121636-       13600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13600)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB413
FEATURES             Location/Qualifiers
     source          1..13600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2302..2961
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2368..2387)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3487..3506
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4675..4927
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4961..4977)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4961..4977)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4974..4996)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4985..5001
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5009..5015,5016..5033,5034..5039))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5054..5075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5160..5334
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5391..6188)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5502..5521)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6112..6131
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6251..6927)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6294..6314)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7122..7146
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7667..8458
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8704..9292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9193..9212
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9446..9463
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9478..9618
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9544..9563)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9704..9726
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9962..10156
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10222..11295)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11724..12353)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcatggcta gtgaagctcg aaaaacaaag aaaaaaatca aagggattca gcaagccact
     3841 gcaggagtct cacaagacac ttcggaaaat cctaacaaaa caatagttcc tgcagcatta
     3901 ccacagctca cccctacctt ggtgtcactg ctggaggtga ttgaacccga ggtgttgtat
     3961 gcaggatatg atagctctgt tccagattca gcatggagaa ttatgaccac actcaacatg
     4021 ttaggtgggc gtcaagtgat tgcagcagtg aaatgggcaa aggcgatacc aggcttcaga
     4081 aacttacacc tggatgacca aatgaccctg ctacagtact catggatgtt tctcatggca
     4141 tttgccctgg gttggagatc atacagacaa tcaagtggaa acctgctctg ctttgctcct
     4201 gatctgatta ttaatgagca gagaatgtct ctaccctgca tgtatgacca atgtaaacac
     4261 atgctgtttg tctcctctga attacaaaga ttgcaggtat cctatgaaga gtatctctgt
     4321 atgaaaacct tactgcttct ctcctcagtt cctaaggaag gtctgaagag ccaagagtta
     4381 tttgatgaga ttcgaatgac ttatatcaaa gagctaggaa aagccatcgt caaaagggaa
     4441 gggaactcca gtcagaactg gcaacggttt taccaactga caaaacttct ggactccatg
     4501 catgaggtgg ttgagaatct ccttacctac tgcttccaga catttttgga taagaccatg
     4561 agtattgaat tcccagagat gttagctgaa atcatcacta atcagatacc aaaatattca
     4621 aatggaaata tcaagaagct gctgtttcat caaaaataga gctcgaattt ccccgatcgt
     4681 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     4741 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     4801 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     4861 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     4921 ctagatcggg aattggttcc ggaaccaatt cgtaatcatg gtcatagctg tttcctgtgt
     4981 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5041 cctggggtgc ctaatgagtg agctaactca cattaggctg aattaggcgc gcctatttct
     5101 gaattcaaca cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg
     5161 atctggattt tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat
     5221 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
     5281 ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag
     5341 cttgtcgatc gactctagct agaggatcga tccgaacccc agagtcccgc tcagaagaac
     5401 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     5461 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     5521 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     5581 cggccatttt ccaccatgat attcggcaag caggcatcgc catgtgtcac gacgagatcc
     5641 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     5701 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     5761 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     5821 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     5881 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     5941 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     6001 tcctggagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     6061 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     6121 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     6181 atccccatgg tcgatcgaca gatctgcgaa agctcgagag agatagattt gtagagagag
     6241 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
     6301 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
     6361 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     6421 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     6481 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     6541 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     6601 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     6661 cgtgctccac catgttcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
     6721 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
     6781 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
     6841 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
     6901 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
     6961 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa
     7021 tacgcaaacc gaattcagaa ataaattcag cctaattcgg cgttaattca gtacattaaa
     7081 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     7141 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     7201 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     7261 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     7321 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     7381 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     7441 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     7501 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     7561 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     7621 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
     7681 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     7741 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     7801 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     7861 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     7921 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     7981 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     8041 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     8101 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     8161 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     8221 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     8281 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     8341 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     8401 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     8461 tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     8521 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     8581 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat
     8641 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8701 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8761 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8821 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     8881 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8941 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9001 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9061 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9121 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9181 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9241 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9301 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9361 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9421 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     9481 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     9541 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     9601 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     9661 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     9721 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
     9781 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
     9841 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
     9901 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
     9961 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
    10021 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
    10081 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
    10141 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
    10201 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
    10261 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
    10321 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
    10381 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
    10441 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
    10501 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
    10561 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
    10621 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
    10681 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
    10741 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
    10801 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
    10861 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
    10921 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
    10981 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
    11041 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
    11101 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
    11161 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
    11221 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
    11281 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
    11341 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
    11401 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
    11461 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
    11521 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
    11581 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
    11641 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
    11701 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
    11761 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
    11821 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
    11881 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
    11941 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
    12001 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
    12061 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
    12121 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
    12181 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
    12241 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
    12301 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
    12361 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
    12421 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
    12481 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
    12541 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
    12601 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
    12661 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
    12721 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
    12781 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
    12841 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
    12901 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
    12961 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
    13021 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
    13081 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
    13141 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
    13201 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
    13261 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
    13321 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
    13381 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
    13441 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
    13501 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
    13561 tggttggcat gcacatacaa atggacgaac ggataaacct
//
