LOCUS       sequence-121640-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zebrafish expression of a photoactivatable fluorescent calcium 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: DF-UAS-spa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..85
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     298..319
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(425..446)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggatctcgcc accatggtcg actcatcacg tcgtaagtgg aataagacag gtcacgcagt
       61 cagagctata ggtcggctga gctcactcga gaacgtctat atcaccgccg acaagcagaa
      121 gaacggcatc aaggcgaact tcaagatccg ccacaacgtg gaggacggca gcgtgcagct
      181 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      241 ccactacctg agccatcagt ccgtgctttc gaaagacccc aacgagaagc gcgatcacat
      301 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
      361 gggcggtacc ggagggagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      421 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtga ggggcgaggg
      481 tgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      541 gcccgtgccc tggcccaccc tcgtgaccac cttcagctac ggcgtgcagt gcttcagccg
      601 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      661 ccaggagcgc accatctcct tcaaggacga cggcacctac aagacccgcg ccgaggtgaa
      721 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      781 cggcaacatc ctggggcaca agctggaata caacctgccg g
//
