LOCUS       sequence-121641-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zebrafish expression of a photoactivatable fluorescent calcium 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: DF-UAS-spa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     272..293
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(399..420)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(633..689)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      701..710
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(717..731,732..740))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(744..776)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(780..797)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      803..812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(818..842)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        842..860
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 acctcggcgc gggtcttgta ggtgccgtcg tccttgaagg agatggtgcg ctcctggacg
       61 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
      121 ctgaagcact gcacgccgta gctgaaggtg gtcacgaggg tgggccaggg cacgggcagc
      181 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt tggtggcatc gccctcaccc
      241 tcgcccctca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      301 accaccccgg tgaacagctc ctcgcccttg ctcaccatgc tccctccggt accgcccttg
      361 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      421 tgatcgcgct tctcgttggg gtctttcgaa agcacggact gatggctcag gtagtggttg
      481 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      541 tgcacgctgc cgtcctccac gttgtggcgg atcttgaagt tcgccttgat gccgttcttc
      601 tgcttgtcgg cggtgatata gacgttctcg agtgagctca gccgacctat agctctgact
      661 gcgtgacctg tcttattcca cttacgacgt gatgagtcga ccatggtggc gagatcctta
      721 tcgtcatcgt cgtacagatc ccgacccatt tgctgtccac cagtcatgct agccatacca
      781 tgatgatgat gatgatgaga acccatggtg gcgcgccagc ctgctttttt gtacaaactt
      841 gaattaaccc tcactaaagg gaaaaaagct ggagctccac cgcggtgg
//
