LOCUS       sequence-121644-        1276 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zebrafish expression of a short photoactivatable fluorescent calcium
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: DF-UAS-sspa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..1276
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..87
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     300..321
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(427..448)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tataggcgcg ccaccatggt cgactcatca cgtcgtaagt ggaataagac aggtcacgca
       61 gtcagagcta taggtcggct gagctcactc gagaacgtct atatcaccgc cgacaagcag
      121 aagaacggca tcaaggcgaa cttcaagatc cgccacaacg tggaggacgg cagcgtgcag
      181 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      241 aaccactacc tgagccatca gtccgtgctt tcgaaagacc ccaacgagaa gcgcgatcac
      301 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      361 aagggcggta ccggagggag catggtgagc aagggcgagg agctgttcac cggggtggtg
      421 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gaggggcgag
      481 ggtgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag
      541 ctgcccgtgc cctggcccac cctcgtgacc accttcagct acggcgtgca gtgcttcagc
      601 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      661 gtccaggagc gcaccatctc cttcaaggac gacggcacct acaagacccg cgccgaggtg
      721 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      781 gacggcaaca tcctggggca caagctggaa tacaacctgc cggaccaact gactgaagag
      841 cagatcgcag aatttaaaga ggaattctcc ctatttgaca aggacgggga tgggacaata
      901 acaaccaagg agctggggac ggtgatgcgg tctctggggc agaaccccac agaagcagag
      961 ctgcaggaca tgatcaatga agtagatgcc gacggtgacg gcacaatcga cttccctgag
     1021 ttcctgacaa tgatggcaag aaaaatgaaa tacagggaca cggaagaaga aattagagaa
     1081 gcgttcggtg tgtttgataa ggatggcaat ggctacatca gtgcagcaga gcttcgccac
     1141 gtgatgacaa accttggaga gaagttaaca gatgaagagg ttgatgaaat gatcagggaa
     1201 gcagacatcg atggggatgg tcaggtaaac tacgaagagt ttgtacaaat gatgacagcg
     1261 aagtgaacgc gttata
//
