LOCUS       sequence-121647-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-H2B-6
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(60..437)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(526..729)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(555..575)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cggagtttga tcctcatgtc tggcttaatc gcactcacca tggtggcgac cggtggatcc
       61 ttagcgctgg tgtacttggt gatggcctta gtaccctcgg acacggcgtg cttggccaac
      121 tccccaggca gcagcaggcg cacggccgtc tggatctccc tggaggtgat ggtcgagcgc
      181 ttgttgtaat gcgccaggcg ggaagcctca cctgcgatgc gctcgaaaat gtcgttcaca
      241 aacgaattca tgatgcccat ggccttggac gaaatgccgg tgtcagggtg gacctgcttc
      301 agaaccttgt acacatagat ggaatagctc tccttgcggc tgcgcttgcg cttcttgccg
      361 cctttcttct gcgccttagt caccgccttc ttggagccct ttttcggggc gggagcagac
      421 ttcgctggct ctggcatggt ggcggtaccg tcgactgcag aattcgaagc ttgagctcga
      481 gatctgagtc cggtagcgct agcggatctg acggttcact aaaccagctc tgcttatata
      541 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
      601 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
      661 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
      721 ccgcatcacc atggt
//
