LOCUS       sequence-121652-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway middle entry vector for full-length attP site for 
            phiC31-mediated transgenesis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI
  TITLE     Site-directed zebrafish transgenesis into single landing sites with 
            the phiC31 integrase system.
  JOURNAL   Dev Dyn. 2013 Aug;242(8):949-63. doi: 10.1002/dvdy.23989. Epub 2013 
            Jul 3.
  PUBMED    23723152
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.23989"; journalName: "Dev Dyn"; date: "2013-08"; 
            volume: "242"; issue: "8"; pages: "949-63"
COMMENT     Sequence Label: pENTR5'-attP (pCM254)
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..94
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    197..296
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     protein_bind    384..507
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(583..602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(584..602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(607..623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(607..623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(725..744)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(803..822)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
       61 ttttttataa tgccaacttt gtatagaaaa gttggctccg aattcgccct tcacctctcg
      121 aggtacctgc agtactgacg gacacaccga agccccggcg gcaaccctca gcggatgccc
      181 cggggcttca cgttttccca ggtcagaagc ggttttcggg agtagtgccc caactggggt
      241 aacctttgag ttctctcagt tgggggcgta gggtcgccga catgacacaa ggggttgtga
      301 ccggggtgga cacgtacgcg ggtgcttacg accgtcagtc gcgcgagcgc gatctagagt
      361 cgtcgacaag ggcgaattcg acccaagttt gtacaaaaaa gttgaacgag aaacgtaaaa
      421 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      481 taaaacacaa catatgcagt cactatgaac caactactta gatggtatta gtgacctgta
      541 gaattaattc gagctctaga gctgcagggc ggccgcgata tcccctatag tgagtcgtat
      601 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
      661 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
      721 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
      781 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
      841 gcgacaatct atcgcttgta tgg
//
