LOCUS       sequence-121658-        1714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High-efficiency blue-shifted cation channelrhodopsin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Govorunova EG, Sineshchekov OA, Li H, Janz R, Spudich JL
  TITLE     Characterization of a highly efficient blue-shifted channelrhodopsin
            from the marine alga Platymonas subcordiformis.
  JOURNAL   J Biol Chem. 2013 Oct 11;288(41):29911-22. doi: 
            10.1074/jbc.M113.505495. Epub 2013 Aug 30.
  PUBMED    23995841
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.505495"; journalName: "J Biol Chem"; date: 
            "2013-10-11- 11"; volume: "288"; issue: "41"; pages: "29911-22"
COMMENT     Sequence Label: pcDNA3.1_PsChR2_EYFP
FEATURES             Location/Qualifiers
     source          1..1714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(979..981,982..984,985..987,988..1701)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MLVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI
                     KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVL
                     LEFVTAAGITLGMDELYK"
     primer_bind     complement(1027..1048)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1288..1307)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1635..1656
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggatccatgg gcttccagct gaacccggag tacctgaacg agacgatcct tctggacgac
       61 tgtaccccca tctacctcaa cgttggccca ctctgggaac agaaagtcgc gcgcgggacg
      121 cagtggttcg gcgtcatcct gtccctcgcc ttccttatct attacatatg gatcacgtac
      181 aaggccacct gcggctggga ggagctctac gtgtgcacaa tcgagttctg caagattgtc
      241 atcgagctgt actttgagtt ctccccgcca gccatgattt accagaccaa cggggaagtc
      301 acgccgtggt tgcgatacgc ggagtggctg ctaacgtgtc cggtgatcct aattcatttg
      361 tccaacatca ccggtttaaa cgacgattac agcggccgca cgatgagttt gataacctca
      421 gatttgggag gcatctgtat ggctgtaacc tctgcactct cgaaaggatg gctcaagtgg
      481 ttgttttttg taatcggctg ctgctacggc gcctccactt tctaccacgc tgctctcatc
      541 tacatcgagt cctactacac catgccacac ggagtctgca aaaatatggt gctcgcgatg
      601 gccgctgtct tcttcacctc ctggttcatg ttcccgggcc tctttctcgc cggtcccgag
      661 ggcacaaacg ctctgtcatg ggccggaagc acaattggtc acacggtggc cgatctgctg
      721 tctaaaaacg catgggggat gatcggacac ttccttcgtc tcgagatcca caaacatatt
      781 atcatccacg gagacgtgcg acgcccgatc acggtgaaca ccctcgggcg tgaggtgacg
      841 gtgtcgtgct ttgtggacaa ggaggaggag gatgaggatg aacgcatctc gactaagact
      901 tacgccaacc gcgcgagctt tatgaagatg aggaacgaca tggagcagcg cggcatccag
      961 acgcgcaagt ctttggaaat gctagtgagc aagggcgagg agctgttcac cggggtggtg
     1021 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1081 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1141 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca gtgcttcgcc
     1201 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1261 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1321 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1381 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     1441 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     1501 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1561 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
     1621 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1681 atggacgagc tgtacaagta acatatgctc taga
//
