LOCUS       sequence-121669-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-TylP
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(744..767)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(777..794)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttggatcct
       61 taatcaccgg ctgcaggcag cggtgcttca cgacgacgca ttgcttcatc ataaacggta
      121 atgctacgtt ctgcgctaaa atccagacga accagaactg ccggaactgc aacgctggtc
      181 atcagatgac gatgcatatc ggtaacacgt tccagcagat ctgcatgacc ggtcagaata
      241 ttgctcagaa tctgtgcacc ggtaaaacca ccaaccagca tacgtgcaac tgcatcaaca
      301 tccagacgag gcagcagttc accaccggct tcggcacgac gcagcagatc acgaccatga
      361 ccaatccatt cttgcatcgg tgcacgacga tccagaccat cacgcggtgc acccggttca
      421 acggtcagac gaacgctacc acgaaccagc ggatcacctt tgctcagcag ctgtgccagc
      481 agcagggttt catcaataat ctgctgcaga accagacgct gttcttcaac aggcggaact
      541 gcacgcagct ggctggtcag aacttcttgt gccagctgtt ctttgctggt aaagtgaaaa
      601 tacagtgcac ctttggtaac accgctacgt ttcagaattt ctgcaatggt ggttgcttca
      661 taacccagtt catcaaaaac ggctgctgct gctgcaacaa tggtacgacg ggtctgtgct
      721 gcacgttctt gacgtgccat atggggcccc tggaacagaa cttccaggcc gctgctgtga
      781 tgatgatgat gatggctgct gcccatggta tatctccttc ttaa
//
