LOCUS       sequence-121686-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite Gateway destination vector for phiC31-mediated 
            transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven 
            EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI
  TITLE     Site-directed zebrafish transgenesis into single landing sites with 
            the phiC31 integrase system.
  JOURNAL   Dev Dyn. 2013 Aug;242(8):949-63. doi: 10.1002/dvdy.23989. Epub 2013 
            Jul 3.
  PUBMED    23723152
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.23989"; journalName: "Dev Dyn"; date: "2013-08"; 
            volume: "242"; issue: "8"; pages: "949-63"
COMMENT     Sequence Label: pDestattB_ubi:EGFP (pCM272)
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..230
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(281..305)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
       61 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      121 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      181 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      241 accccggtga acagctcctc gcccttgctc accatggtcc agcctgcttt tttgtacaaa
      301 cttgggtcga attcgccctt aaaggatccc tgtaaacaaa ttcaaagtaa gattagcaat
      361 cacacatttc catttaaaaa atactacaat gttctagcca ataatgtatg ttttgcactt
      421 ctcccatgtt aaacgagata gttcacccga aaatgaaatt ttactttgca tttggtcacc
      481 cttaaatgtt acaaaccttt aaaataatag aaaactattg actatacttg tagagaaaaa
      541 taaatactat ccaaaacaat ggctgacagt ggactataga atgcatttta cctgcagcaa
      601 aattgtccaa atgatcctat atgaaaattt agggtcttac ctagtgaaat gatagctcat
      661 tttgcacaag ctctgagatt tggctttgtg catcatgaaa tctaattatg tcatgcattt
      721 ccaaagcatt ggccatttta aacaaactgc acatctgtgc agtttacatt gttttaa
//
