LOCUS       sequence-121691-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses dsRed and shRNA to knock down adra2a receptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Zhang Z, Ferretti V, Guntan I, Moro A, Steinberg EA, Ye Z, Zecharia 
            AY, Yu X, Vyssotski AL, Brickley SG, Yustos R, Pillidge ZE, Harding 
            EC, Wisden W, Franks NP
  TITLE     Neuronal ensembles sufficient for recovery sleep and the sedative 
            actions of alpha2 adrenergic agonists.
  JOURNAL   Nat Neurosci. 2015 Apr;18(4):553-61. doi: 10.1038/nn.3957. Epub 2015
            Feb 23.
  PUBMED    25706476
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3957"; 
            journalName: "Nat Neurosci"; date: "2015-04"; volume: "18"; issue: 
            "4"; pages: "553-61"
COMMENT     Sequence Label: AAV-actin prom-dsRed-adra2a.1227.shRNA
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..87
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    158..191
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(217..246)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atccagaggt tgattatcga taagctgtta aagcttgaac ctcttcgagg gacataactt
       61 cgtataatgt atgctatacg aagttatggg tcgatggtga gatctggact agagggtcga
      121 tggtgatgct tggatcaaaa taggaagaat cccaatgata acttcgtata aagtatccta
      181 tacgaagtta tgatttaatt aagtttaaac acatgtcaga tcctcttcgg agatcagctt
      241 ctgttcggag ccgcgtggca gggagccacc gcccaaacgc gcgagatccg aatttctgcc
      301 attcatccgc ttattatcac ttattcaggc gtagcaacca ggcgtttaag ggcaccaata
      361 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
      421 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
      481 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
      541 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
      601 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
      661 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
      721 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
      781 ccatatcacc agctcaccgt ctttcattgc c
//
