LOCUS       sequence-121692-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubule-Binding Protein, Excitation: 448 / 473, 
            Emission: 500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-RatLC3
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..371
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     CDS             393..815
                     /codon_start=1
                     /gene="Map1lc3b"
                     /product="rat LC3 protein"
                     /label=LC3
                     /note="Atg8 family member found on autophagosomes"
                     /translation="PSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPV
                     LDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDE
                     DGFLYMVYASQETFGTALAVTYMSALKATATGREPCL"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggaga
       61 aaagtttcgt gataactgat ccacggctgc cagacaatcc catcatcttc gcatccgatg
      121 gcttcctgga gctgaccgag tattccagag aggagatcct gggccgcaat ggccgctttc
      181 tgcagggacc agagacagac caggccacag tgcagaagat tcgcgatgcc attagagatc
      241 agcgcgagat taccgtgcag ctgataaact acacaaaaag cgggaagaaa ttctggaacc
      301 tcctgcacct ccagcccatg agggaccaga agggtgagct ccagtatttc atcggagtgc
      361 agctggatgg atccggactc agatcctcta gaccgtccga gaagaccttc aaacagcgcc
      421 ggagcttcga acaaagagtg gaagatgtcc ggctcatccg ggagcagcac cccaccaaga
      481 tcccagtgat tatagagcga tacaagggtg agaagcagct gcccgtcctg gacaagacca
      541 agttccttgt acctgatcac gtgaatatga gcgaactcat caagataatt agaaggcgcc
      601 tgcagctcaa tgctaaccaa gccttcttcc tcctggtgaa tgggcacagc atggtgagtg
      661 tgtccacacc catctctgaa gtgtacgaga gcgagagaga tgaagacggc ttcctgtaca
      721 tggtctatgc ctcccaggag acgttcggga cagcactggc tgttacatac atgtcagctc
      781 tgaaggcaac agcaacagga agagagccat gcttgtgaca gacatacagc cacttccaac
      841 taaagcaagc ctctgctccc tgctacctgc atggagccca ctgtgacac
//
