LOCUS       sequence-121693-         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miniSOG fused to N terminal 29 aa of human Cox8a for targeting to 
            the mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Qi YB, Garren EJ, Shu X, Tsien RY, Jin Y
  TITLE     Photo-inducible cell ablation in Caenorhabditis elegans using the 
            genetically encoded singlet oxygen generating protein miniSOG.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 8;109(19):7499-504. doi: 
            10.1073/pnas.1204096109. Epub 2012 Apr 24.
  PUBMED    22532663
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1204096109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-05-8- 8"; volume: "109"; issue: "19"; pages: "7499-504"
COMMENT     Sequence Label: pCZGY1026 COX8aN::miniSOG in pENTR[1-2]
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(98..418)
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     regulatory      415..424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(452..526)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     protein_bind    complement(527..626)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
       61 tttcttataa tgccaacttt gtacaagaaa gctgggttta tccatccagc tgcactccga
      121 tgaaatactg gagctcaccc ttctggtccc tcatgggctg gaggtgcagg aggttccaga
      181 atttcttccc gctttttgtg tagtttatca gctgcacggt aatctcgcgc tgatctctaa
      241 tggcatcgcg aatcttctgc actgtggcct ggtctgtctc tggtccctgc agaaagcggc
      301 cattgcggcc caggatctcc tctctggaat actcggtcag ctccaggaag ccatcggatg
      361 cgaagatgat gggattgtct ggcagccgtg gatcagttat cacgaaactt ttctccatgg
      421 tggcgaccgg tggatccccc aacgaatgga tcttggcgcg cggcactggg agccgccggg
      481 ccgagcctgt caagccccgc agcagcagcg gcgtcaggac ggacatagcc tgcttttttg
      541 tacaaagttg gcattataaa aaagcattgc tcatcaattt gttgcaacga acaggtcact
      601 atcagtcaaa ataaaatcat tatttgg
//
