LOCUS       sequence-121695-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for 
            targeting to the mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Qi YB, Garren EJ, Shu X, Tsien RY, Jin Y
  TITLE     Photo-inducible cell ablation in Caenorhabditis elegans using the 
            genetically encoded singlet oxygen generating protein miniSOG.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 8;109(19):7499-504. doi: 
            10.1073/pnas.1204096109. Epub 2012 Apr 24.
  PUBMED    22532663
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1204096109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-05-8- 8"; volume: "109"; issue: "19"; pages: "7499-504"
COMMENT     Sequence Label: pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(113..427)
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="EKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQGP
                     ETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQLD
                     G"
     protein_bind    complement(599..698)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
       61 tttcttataa tgccaacttt gtacaagaaa gctgggttta tccggaagat cctccatcca
      121 gctgcactcc gatgaaatac tggagctcac ccttctggtc cctcatgggc tggaggtgca
      181 ggaggttcca gaatttcttc ccgctttttg tgtagtttat cagctgcacg gtaatctcgc
      241 gctgatctct aatggcatcg cgaatcttct gcactgtggc ctggtctgtc tctggtccct
      301 gcagaaagcg gccattgcgg cccaggatct cctctctgga atactcggtc agctccagga
      361 agccatcgga tgcgaagatg atgggattgt ctggcagccg tggatcagtt atcacgaaac
      421 ttttctctcc ggatgctcca gcctgggcac gtctcttttg cctaatcttg tccttgtagt
      481 ctggagcgtt gattctctta tgatcgaagt aaatgcagta gccgaggaaa gcggctccag
      541 caattccagc agccaaaacg acgtttgatt tgttgaaacc aagaattgtg tccgacatag
      601 cctgcttttt tgtacaaagt tggcattata aaaaagcatt gctcatcaat ttgttgcaac
      661 gaacaggtca ctatcagtca aaataaaatc attatttggg gcc
//
