LOCUS       sequence-121700-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBW400hrpL-gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Wang B, Kitney RI, Joly N, Buck M
  TITLE     Engineering modular and orthogonal genetic logic gates for robust 
            digital-like synthetic biology.
  JOURNAL   Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
  PUBMED    22009040
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-10-18"; volume: "2:508. doi"; pages: " 
            10.1038/ncomms1516"
COMMENT     Sequence Label: pBW400hrpL-gfp
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    14..35
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             255..266
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(303..331)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     926..946
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aggatgattt ctggaattcg cggccgcttc tagaggccgg attatgtccg ctgagtgggt
       61 cacggtcccg gatcagttcc cttgcgaagc tgaccgatgt ttttgtgcca aaagctgttg
      121 tggcaaaaaa cggtttgcgc aaagttttgt attacaaaga atttcacatt ttaaaatatc
      181 tttataaatc aatcagttat ttctattttt aagctggcat ggttatcgct atagggcttg
      241 tactactaga gattaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg
      301 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
      361 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
      421 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
      481 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
      541 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
      601 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
      661 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
      721 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
      781 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
      841 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
      901 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctggga
//
