LOCUS       sequence-121701-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBW400hrpL-gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Wang B, Kitney RI, Joly N, Buck M
  TITLE     Engineering modular and orthogonal genetic logic gates for robust 
            digital-like synthetic biology.
  JOURNAL   Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
  PUBMED    22009040
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-10-18"; volume: "2:508. doi"; pages: " 
            10.1038/ncomms1516"
COMMENT     Sequence Label: pBW400hrpL-gfp
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    41..61
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(68..95)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(111..182)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(205..918)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(245..265)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     860..888
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaaagggcag ggtggtgaca ccttgccctt ttttgccgga ctgcagcggc cgctactagt
       61 atataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc
      121 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
      181 tgatgcctgg ctctagtatt attatttgta tagttcatcc atgccatgtg taatcccagc
      241 agctgttaca aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag
      301 ggcagattgt gtggacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg
      361 agtattttgt tgataatggt ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat
      421 tttgaagtta actttgattc cattcttttg tttgtctgcc atgatgtata cattgtgtga
      481 gttatagttg tattccaatt tgtgtccaag aatgtttcca tcttctttaa aatcaatacc
      541 ttttaactcg attctattaa caagggtatc accttcaaac ttgacttcag cacgtgtctt
      601 gtagttcccg tcatctttga aaaatatagt tctttcctgt acataacctt cgggcatggc
      661 actcttgaaa aagtcatgct gtttcatatg atctgggtat ctcgcaaagc attgaacacc
      721 ataaccgaaa gtagtgacaa gtgttggcca tggaacaggt agttttccag tagtgcaaat
      781 aaatttaagg gtaagttttc cgtatgttgc atcaccttca ccctctccac tgacagaaaa
      841 tttgtgccca ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag
      901 ttcttctcct ttacgcatct agtatttc
//
