LOCUS       sequence-121703-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conjugally transferable red recombinogenic plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Hossain MJ, Thurlow CM, Sun D, Nasrin S, Liles MR
  TITLE     Genome modifications and cloning using a conjugally transferable 
            recombineering system.
  JOURNAL   Biotechnol Rep (Amst). 2015 Aug 28;8:24-35. doi: 
            10.1016/j.btre.2015.08.005. eCollection 2015 Dec.
  PUBMED    28352570
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Rep (Amst)."; date: "2015-08-28"; volume: "8:24-35. doi"; pages: " 
            10.1016/j.btre.2015.08.005. eCollection 2015 Dec"
COMMENT     Sequence Label: pMJH46
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     182..201
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(268..370)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg
       61 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
      121 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
      181 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
      241 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
      301 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      361 gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg
      421 gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt attcgcgata
      481 agctcatgga gcggcgtaac cgtcgcacag gaaggacaga gaaagcgcgg atctgggaag
      541 tgacggacag aacggtcagg acctggattg gggaggcggt tgccgccgct gctgctgacg
      601 gtgtgacgtt ctctgttccg gtcacaccac atacgttccg ccattcctat gcgatgcaca
      661 tgctgtatgc cggtataccg ctgaaagttc tgcaaagcct gatgggacat aagtccatca
      721 gttcaacgga agtctacacg cagcagtcag tcacgataat gatgtggtct gtccttttac
      781 agccagtagt gctcgc
//
