LOCUS       sequence-121706-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway middle entry vector for full-length attP site for 
            phiC31-mediated transgenesis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI
  TITLE     Site-directed zebrafish transgenesis into single landing sites with 
            the phiC31 integrase system.
  JOURNAL   Dev Dyn. 2013 Aug;242(8):949-63. doi: 10.1002/dvdy.23989. Epub 2013 
            Jul 3.
  PUBMED    23723152
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.23989"; journalName: "Dev Dyn"; date: "2013-08"; 
            volume: "242"; issue: "8"; pages: "949-63"
COMMENT     Sequence Label: pENTR5'-attP (pCM254)
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(62..185)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    273..372
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     protein_bind    complement(475..570)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(610..627)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(610..626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(619..641)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      690..776
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cctgcagctc tagagctcga attaattcta caggtcacta ataccatcta agtagttggt
       61 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
      121 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt ttttgtacaa
      181 acttgggtcg aattcgccct tgtcgacgac tctagatcgc gctcgcgcga ctgacggtcg
      241 taagcacccg cgtacgtgtc caccccggtc acaacccctt gtgtcatgtc ggcgacccta
      301 cgcccccaac tgagagaact caaaggttac cccagttggg gcactactcc cgaaaaccgc
      361 ttctgacctg ggaaaacgtg aagccccggg gcatccgctg agggttgccg ccggggcttc
      421 ggtgtgtccg tcagtactgc aggtacctcg agaggtgaag ggcgaattcg gagccaactt
      481 ttctatacaa agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg
      541 tcactatcag tcaaaataaa atcattattt ggagctccat ggtagcgtta actcgggccc
      601 gagcttaaga ctggccgtcg ttttacaacg tcgtgactgg gaaaacatcc atgctagcgt
      661 taacgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcggaagact
      721 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
      781 cgggagcgga tt
//
