LOCUS       sequence-121707-       10254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conjugally transferable red recombinogenic plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Hossain MJ, Thurlow CM, Sun D, Nasrin S, Liles MR
  TITLE     Genome modifications and cloning using a conjugally transferable 
            recombineering system.
  JOURNAL   Biotechnol Rep (Amst). 2015 Aug 28;8:24-35. doi: 
            10.1016/j.btre.2015.08.005. eCollection 2015 Dec.
  PUBMED    28352570
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Rep (Amst)."; date: "2015-08-28"; volume: "8:24-35. doi"; pages: " 
            10.1016/j.btre.2015.08.005. eCollection 2015 Dec"
COMMENT     Sequence Label: pMJH65
FEATURES             Location/Qualifiers
     source          1..10254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(11..889)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        916..1200
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1123..1142
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             1227..1235
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1244..1660
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             1666..2451
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             2448..3128
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      3129..3373
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     oriT            4236..4345
                     /note="incP origin of transfer"
     CDS             4378..4749
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             complement(5689..6879)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     6508..6527
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(6927..6932,6933..6949,6950..6955))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             complement(7421..8371)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      8419..8641
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(join(9263..10054,10055..10123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9886..9905
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10124..10228)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
       61 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
      121 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
      181 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
      241 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
      301 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
      361 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
      421 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
      481 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
      541 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
      601 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
      661 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
      721 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
      781 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
      841 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
      901 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
      961 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     1021 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     1081 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     1141 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     1201 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga
     1261 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc
     1321 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat
     1381 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga
     1441 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc
     1501 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt
     1561 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac
     1621 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc
     1681 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa
     1741 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc
     1801 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc
     1861 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc
     1921 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca
     1981 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa
     2041 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg
     2101 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga
     2161 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact
     2221 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt
     2281 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc
     2341 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa
     2401 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca
     2461 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc
     2521 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc
     2581 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg
     2641 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg
     2701 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga
     2761 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg
     2821 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg
     2881 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga
     2941 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc
     3001 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg
     3061 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat
     3121 ggcgatgacg catcctcacg ataatatccg ggtaggcgca atcactttcg tctactccgt
     3181 tacaaagcga ggctgggtat ttcccggcct ttctgttatc cgaaatccac tgaaagcaca
     3241 gcggctggct gaggagataa ataataaacg aggggctgta tgcacaaagc atcttctgtt
     3301 gagttaagaa cgagtatcga gatggcacat agccttgctc aaattggaat caggtttgtg
     3361 ccaataccag tagaaacaga cgaagaatcc atgatcaccg ctaacctgtc ttttaacctg
     3421 cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac
     3481 cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc taacgcgggc
     3541 ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca
     3601 ggggatctcg agggatcctt tttgtccggt gttgggttga aggtgaagcc ggtcggggcc
     3661 gcagcggggg ccggcttttc agccttgccc ccctgcttcg gccgccgtgg ctccggcgtc
     3721 ttgggtgccg gcgcgggttc cgcagccttg gcctgcggtg cgggcacatc ggcgggcttg
     3781 gccttgatgt gccgcctggc gtgcgagcgg aacgtctcgt aggagaactt gaccttcccc
     3841 gtttcccgca tgtgctccca aatggtgacg agcgcatagc cggacgctaa cgccgcctcg
     3901 acatccgccc tcaccgccag gaacgcaacc gcagcctcat cacgccggcg cttcttggcc
     3961 gcgcgggatt caacccactc ggccagctcg tcggtgtagc tctttggcat cgtctctcgc
     4021 ctgtcccctc agttcagtaa tttcctgcat ttgcctgttt ccagtcggta gatattccac
     4081 aaaacagcag ggaagcagcg cttttccgct gcataaccct gcttcggggt cattatagcg
     4141 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
     4201 gtagactttc cttggtgtac ccaacggcgt cagccgggca ggataggtga agtaggccca
     4261 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     4321 aatcctgctc tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg
     4381 gctgatgaaa ccaagccaac caggaagggc agcccaccta tcaaggtgta ctgccttcca
     4441 gacgaacgaa gagcgattga ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac
     4501 ctgctggccg tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc
     4561 gagctggccc gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc
     4621 accgacgacc cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag
     4681 atcgaagaga agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca
     4741 gagccatgac ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt
     4801 ccccatgcgc tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga
     4861 gcaaggcaag accgagcgcc tgggtcacgt gcgcgtcacg aactgcgagg caatcaccct
     4921 gcccgctgtc atggccgagg tgatggcgac ccagcacggc aacacccgtt ccgaggccga
     4981 caagacctat cacctgctgg ttagcttccg cgcgggagag aagcccgacg cggagacgtt
     5041 gcgcgcgatt gaggaccgca tctgcgctgg gcttggcttc gccgagcatc agcgcgtcag
     5101 tgccgtgcat cacgacaccg acaacctgca catccatatc gccatcaaca agattcaccc
     5161 gacccgaaac accatccatg agccgtatcg ggcctaccgc gccctcgctg acctctgcgc
     5221 gacgctcgaa cgggactacg ggcttgagcg tgacaatcac gaaacgcggc agcgcgtttc
     5281 cgagaaccgc gcgaacgaca tggagcggca cgcgggcgtg gaaagcctgg tcggctgctc
     5341 gagggcttcg ccctgtcgct cgactgcggc gagcactact ggctgtaaaa ggacagacca
     5401 catcattatc gtgactgact gctgcgtgta gacttccgtt gaactgatgg acttatgtcc
     5461 catcaggctt tgcagaactt tcagcggtag acaggagcac gatcatgcgc acccgtggcc
     5521 aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg
     5581 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
     5641 caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt
     5701 ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc
     5761 tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat
     5821 cagcggtcca atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc
     5881 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     5941 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     6001 caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa
     6061 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     6121 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     6181 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc
     6241 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     6301 gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     6361 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
     6421 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     6481 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     6541 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcc acaggacggg
     6601 tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg
     6661 gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa
     6721 cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc
     6781 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac
     6841 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta
     6901 gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg
     6961 agaattacaa cttatatcgt atggggctga cttcaggtgc tacacatggg caaatattat
     7021 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     7081 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     7141 ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga
     7201 ataagtgata ataagcggat gaatggcaga aattcgatga taagctgtca aacatgagaa
     7261 ttggtcgacg gcccgggcgg ccgcaagggg ttcgcgttgg ccgattcatt agatcatggg
     7321 tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt tgtttgttag
     7381 tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag
     7441 ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga
     7501 gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt
     7561 ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt acgtaggtag gaatctgatg
     7621 taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta
     7681 cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc
     7741 gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca
     7801 aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact
     7861 taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt
     7921 ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca
     7981 gattatattt tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa
     8041 ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc
     8101 ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag
     8161 ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag
     8221 tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca
     8281 cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt
     8341 catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat
     8401 cactatacca attgagatgg gctagtcaat gataattact agtccttttc ctttgagttg
     8461 tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct
     8521 ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt
     8581 atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact
     8641 cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc
     8701 tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc tcggttgcgg
     8761 ccgcaatcgg gcaaatcgct gaatattcct tttgtctccg accatcaggc acctgagtcg
     8821 ctgtcttttt cgtgacattc agttcgctgc gctcacggct ctggcagtga atgggggtaa
     8881 atggcactac aggcgccttt tatggattca tgcaaggaaa ctacccataa tacaagaaaa
     8941 gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg ctatgtggtg ctatctgact
     9001 ttttgctgtt cagcagttcc tgccctctga ttttccagtc tgaccacttc ggattatccc
     9061 gtgacaggtc attcagactg gctaatgcac ccagtaaggc agcggtatca tcaacggggt
     9121 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9181 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9241 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9301 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9361 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9421 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9481 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9541 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9601 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9661 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9721 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9781 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9841 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9901 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9961 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10021 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10081 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10141 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10201 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctg
//
