LOCUS       sequence-121710-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBAD18-cm encoding rbs33-hrpS-ter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Wang B, Kitney RI, Joly N, Buck M
  TITLE     Engineering modular and orthogonal genetic logic gates for robust 
            digital-like synthetic biology.
  JOURNAL   Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
  PUBMED    22009040
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-10-18"; volume: "2:508. doi"; pages: " 
            10.1038/ncomms1516"
COMMENT     Sequence Label: pBW213ara-hrpS
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    45..65
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(72..99)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(115..186)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagag gatccccggg taccctgcag cggccgctac
       61 tagtatataa acgcagaaag gcccacccga aggtgagcca gtgtgactct agtagagagc
      121 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
      181 tatttgatgc ctggttatta gatctgcaat tctttgatgc gtcgatagag cgtacggcgt
      241 ggcacgtcca gttccaggct taccgaatcc acacaattgt cgtggcgctt gagcgactcc
      301 tgaatcaggg ctttttcgat cactcgcaat tgcgatttga gcccacaggc caagtgctct
      361 tcgccctgcg gctcggcgcc cagcaagggg aaacccagca catggcgttt ggctgccgcc
      421 ttgagctcac ggatattgcc gggccagtcg tggcccagca gcactttgtg cagcagtggg
      481 caaacatcgg gaacgggaac accgagctcc ctcgcggcgg cggccgtaaa acgtgtgaac
      541 aggggaacta tgcgatcaga ctggttacgt agcggaggaa gcttgagtgt caggacgttc
      601 aggcgaaaat acagatcgcg acgaaactgc ccccgctcga cggcgtcgtc cagcgagcat
      661 tgggcggagg cgatcacgca gatatccagc ttgatcgtcg acgtcgaacc cagccgttca
      721 agcgctcggg tttccagcac cctcagcaat ttggcttgca ggctcagcgg catgctatcg
      781 atctcatcca ggtacagcgt gccgccctgc gccgcttcga cataaccgac tctggagcga
      841 tcagcgccgg tgtaggcacc gctgaccacg ccgaataact cgctctcggc gagggactcc
      901 ggaatggccg cgcaattcat cgccaccagg cgccctttgc ggcctgacat ctcatgaatc
      961 cgtcgggcaa tcgtgtcttt gcccgtgccg gtctcacccg atagcagcac gtcgataccc
     1021 agttgcgaaa tactttcggc aactatcccc a
//
