LOCUS       sequence-121720-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N1 invertor plasmid for two-strain system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Chen Y, Kim JK, Hirning AJ, Josic K, Bennett MR
  TITLE     SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic 
            microbial consortium.
  JOURNAL   Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
  PUBMED    26315440
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-08-28- 28"; volume: "349"; 
            issue: "6251"; pages: "986-9"
COMMENT     Sequence Label: pC235
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    299..315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     304..326
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             517..546
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    700..718
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    730..748
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tcttccagca cttgttgacg gaagccgaac tctttggacc cgtaagccag cgtcatgact
       61 gaacgcttag tcacactgcg agtaacaccg taagccagcc attgaccagc cagtgcctta
      121 gtgcccagct tgactttctc agagatttca ccagtgttct catcggtcac ggtaactact
      181 tcgttatcgg tcccattgat tgcgtctgct tgtagaatct cgttgacttt cttagcaaca
      241 atcccgtaga tgtcctgaac ggtttcacta ggaagcaagt taagacgtct gtgtggaatt
      301 gtgagcggat aacaatttca cacagggccc tcggacaccg aggagaatgt caagaggcga
      361 acacacaacg tcttggagcg ccagaggagg aacgagctaa aacggagctt ttttgccctg
      421 cgtgaccaga tcccggagtt ggaaaacaat gaaaaggccc ccaaggtagt tatccttaaa
      481 aaagccacag catacatcct gtccgtccaa gcagaggagc aaaagctcat ttctgaagag
      541 gacttgttgc ggaaacgacg agaacagttg aaacacaaac ttgaacagct acggaactct
      601 tgtgcgtggc gcatgtcgtt atcgcaaaac cgctgcacac ttttgcgcga catgctctga
      661 tccccctcat ctgggggggc ctatctgagg gaatttactt ccctatcagt gatagagata
      721 atgagcacat ccctatcagt gatagagatg catgccactt aaaaaggaga tcaacaatga
      781 aagcaatttt
//
