LOCUS       sequence-121732-       13890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB314
FEATURES             Location/Qualifiers
     source          1..13890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3797..4049
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4083..4099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4083..4099)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4096..4118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4107..4123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4131..4137,4138..4155,4156..4161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4176..4197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4282..4457
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6541..7217)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    7412..7436
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7957..8748
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8994..9582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9483..9502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9736..9753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9768..9908
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9834..9853)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9994..10016
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10252..10446
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10512..11585)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12014..12643)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcatggc tagtgaagct cgaaaaacaa agaaaaaaat
     2941 caaagggatt cagcaagcca ctgcaggagt ctcacaagac acttcggaaa atcctaacaa
     3001 aacaatagtt cctgcagcat taccacagct cacccctacc ttggtgtcac tgctggaggt
     3061 gattgaaccc gaggtgttgt atgcaggata tgatagctct gttccagatt cagcatggag
     3121 aattatgacc acactcaaca tgttaggtgg gcgtcaagtg attgcagcag tgaaatgggc
     3181 aaaggcgata ccaggcttca gaaacttaca cctggatgac caaatgaccc tgctacagta
     3241 ctcatggatg tttctcatgg catttgccct gggttggaga tcatacagac aatcaagtgg
     3301 aaacctgctc tgctttgctc ctgatctgat tattaatgag cagagaatgt ctctaccctg
     3361 catgtatgac caatgtaaac acatgctgtt tgtctcctct gaattacaaa gattgcaggt
     3421 atcctatgaa gagtatctct gtatgaaaac cttactgctt ctctcctcag ttcctaagga
     3481 aggtctgaag agccaagagt tatttgatga gattcgaatg acttatatca aagagctagg
     3541 aaaagccatc gtcaaaaggg aagggaactc cagtcagaac tggcaacggt tttaccaact
     3601 gacaaaactt ctggactcca tgcatgaggt ggttgagaat ctccttacct actgcttcca
     3661 gacatttttg gataagacca tgagtattga attcccagag atgttagctg aaatcatcac
     3721 taatcagata ccaaaatatt caaatggaaa tatcaagaag ctgctgtttc atcaaaaata
     3781 gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     3841 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     3901 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     3961 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     4021 gcgcgcggtg tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca
     4081 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     4141 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc
     4201 tgaattaggc gcgcctattt ctgaattcaa cacattgcgg acgtttttaa tgtactgaat
     4261 taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta
     4321 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
     4381 ctcaacacat gagcgaaacc ctataggaac cctaaattcc cttatctggg aactactcac
     4441 acattattat ggagaaactc gaagttagta tatagtcctt ccatcaccct ctgtgataat
     4501 ctccttaaaa cttgctcccc caggaatcat aggcagaacg tgctcctggt ggggaactat
     4561 cacatccagt aaataaggac cgggagaatc tagcatagtt tgaattgctg ctcttagttc
     4621 atccttcttc accacacgcg catttggcac cttgaaaccg tctgcaactg tacagaagtc
     4681 ggggtagatc tgctcctcgt gatctggatc acctaagtaa gtatgtgctc tgttggcctt
     4741 gtagaatcta tcctccaatt gtacgaccat tcctagatgt tggttgttta agatcatgat
     4801 cttcacgggt aacttctcca caaaaattgt agccaactcc tgtacgttca tcacaaagct
     4861 accatcgcca tcgatgtcga tcacaggaat atctggttga gttgcggcag cccccaaagc
     4921 agaaggaagt ccgaatccca tggctccaag acctccagat gtcaaccaac gccgcggttc
     4981 gttgtacttg aaccactggg ctgcccacat ttgatgttgg ccaacaccag tagatatgat
     5041 ggcatttcca ccagtgagtt cgtacagcgt ctgtatggca gattgaggag caatggcatc
     5101 ctcgaatacg ggaaacttca ttggccattt ctgcttttgt tcttctagtt ctgccaccca
     5161 gttggagaag tcgaaagtcc tcttttgact cttcaagagg gcattgattt cacttagcgc
     5221 aggtttcaca tcagcacaca tggacacatg gggctgtttg ttctttccaa tttcagcagg
     5281 gtcaatatcg atatgaacga tgctggacct gctagcaaaa gtctccagtt taccagtcac
     5341 tcgatcgtcg aaacgcactc caaatgccaa caacagatct gcttggtcca cggcgtagtt
     5401 ggcgtaaaca gttccatgca ttccaagcat tttcaaactt aatggatcag actcgggata
     5461 cgctcccaaa cccattaaag tcgatgtgat aggaattccg gtaagctcga caaattccct
     5521 caactcttcg ctggcgtgat tgcaacctcc tccagaatac actacaggct tcttagaact
     5581 agaaatgagt cttataatct gctcgattaa cgttggttcc ggtggccgag gcagtcgttg
     5641 taagtagccc ttcagttgca ttggctgaga ccaatctgga atagccattt gctgttgcac
     5701 gtccttggga atatctacca agactgggcc cggcctacct gaagctgcca aaaagaaagc
     5761 ctccctgatt acgcgaggga tgtcatcaat gttcataaca aggtaattgt gcttggtgat
     5821 cgatctcgta acctccacaa ttggagtttc ttgaaaagca tcggtaccaa tcatcctact
     5881 ggatacttga cctgtaatgg ccactagtgg cacactgtcc aagagggcat cagcgagccc
     5941 ggtaacaaga ttggtcgctc caggaccgga tgtggctata caaaccccta ctcgtccaga
     6001 cgatttagca taaccttcag cagcgaaaat ctccccctgc tcgtggcgac agagaatgtt
     6061 gcggatgctg ctggaacgtg taagagcctg gtgaatctcc atagacgctc cgccgggata
     6121 agcgaacacc gttctaatcc cttctcgctc aagagcctcc accaaaatgt cggctccttt
     6181 gcggggctcg tcatccttgt agcgagactg aaagtcggac ggggttggtg caacgagtga
     6241 cttcccgctg ctaagccgtg gtccactcac atcgactgca gactggatct tctcgctaac
     6301 agcttgggct ttcacttcca gtgaacgctc agtcttccga gaggtgttgt atgcatgtcc
     6361 cctcagagcg gctcccgaga agaaaccact cctcaccttt agctctttag ctcccttgca
     6421 acaagacctt gccgatggct gcaccttctc caggctcgct atgctcaaag gcacagctgc
     6481 gcatccacta attgacgcca tgctcgagag agatagattt gtagagagag actggtgatt
     6541 tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
     6601 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
     6661 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     6721 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaattatg
     6781 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
     6841 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
     6901 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
     6961 catgttcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
     7021 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga
     7081 tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc
     7141 ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
     7201 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg
     7261 agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa tacgcaaacc
     7321 gaattcagaa ataaattcag cctaattcgg cgttaattca gtacattaaa aacgtccgca
     7381 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
     7441 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     7501 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     7561 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     7621 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg
     7681 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     7741 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     7801 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     7861 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     7921 cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt gatcgccgaa
     7981 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     8041 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     8101 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     8161 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     8221 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     8281 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     8341 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     8401 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     8461 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     8521 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     8581 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     8641 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     8701 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctagctaga
     8761 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     8821 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     8881 gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt
     8941 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9001 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9061 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9121 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     9181 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9241 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9301 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9361 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9421 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9541 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9601 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9661 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9721 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     9781 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     9841 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     9901 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     9961 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
    10021 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt
    10081 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
    10141 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
    10201 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag
    10261 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
    10321 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
    10381 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
    10441 tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
    10501 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
    10561 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
    10621 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
    10681 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
    10741 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
    10801 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc
    10861 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct
    10921 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca
    10981 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca
    11041 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc
    11101 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc
    11161 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg
    11221 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga
    11281 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc
    11341 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg
    11401 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca
    11461 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc
    11521 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg
    11581 ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc
    11641 agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg
    11701 tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca
    11761 tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta
    11821 atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac
    11881 tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg
    11941 ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct
    12001 ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc
    12061 gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg
    12121 ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg
    12181 cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg
    12241 cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg
    12301 tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga
    12361 tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc
    12421 ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg
    12481 gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta
    12541 acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct
    12601 tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat
    12661 ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc
    12721 aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg
    12781 ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga
    12841 gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc
    12901 ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg
    12961 cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca
    13021 ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg
    13081 gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg
    13141 atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc
    13201 acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc
    13261 tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg
    13321 tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca
    13381 atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg
    13441 tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg
    13501 gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg
    13561 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
    13621 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
    13681 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
    13741 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
    13801 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
    13861 gcacatacaa atggacgaac ggataaacct
//
