LOCUS       sequence-121733-       12722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12722)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB414
FEATURES             Location/Qualifiers
     source          1..12722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3797..4049
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4083..4099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4083..4099)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4096..4118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4107..4123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4131..4137,4138..4155,4156..4161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4176..4197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4282..4456
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4513..5310)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4624..4643)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5234..5253
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5373..6049)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    6244..6268
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6789..7580
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7826..8414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8315..8334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8568..8585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8600..8740
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8666..8685)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8826..8848
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9084..9278
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9344..10417)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10846..11475)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcatggc tagtgaagct cgaaaaacaa agaaaaaaat
     2941 caaagggatt cagcaagcca ctgcaggagt ctcacaagac acttcggaaa atcctaacaa
     3001 aacaatagtt cctgcagcat taccacagct cacccctacc ttggtgtcac tgctggaggt
     3061 gattgaaccc gaggtgttgt atgcaggata tgatagctct gttccagatt cagcatggag
     3121 aattatgacc acactcaaca tgttaggtgg gcgtcaagtg attgcagcag tgaaatgggc
     3181 aaaggcgata ccaggcttca gaaacttaca cctggatgac caaatgaccc tgctacagta
     3241 ctcatggatg tttctcatgg catttgccct gggttggaga tcatacagac aatcaagtgg
     3301 aaacctgctc tgctttgctc ctgatctgat tattaatgag cagagaatgt ctctaccctg
     3361 catgtatgac caatgtaaac acatgctgtt tgtctcctct gaattacaaa gattgcaggt
     3421 atcctatgaa gagtatctct gtatgaaaac cttactgctt ctctcctcag ttcctaagga
     3481 aggtctgaag agccaagagt tatttgatga gattcgaatg acttatatca aagagctagg
     3541 aaaagccatc gtcaaaaggg aagggaactc cagtcagaac tggcaacggt tttaccaact
     3601 gacaaaactt ctggactcca tgcatgaggt ggttgagaat ctccttacct actgcttcca
     3661 gacatttttg gataagacca tgagtattga attcccagag atgttagctg aaatcatcac
     3721 taatcagata ccaaaatatt caaatggaaa tatcaagaag ctgctgtttc atcaaaaata
     3781 gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     3841 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     3901 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     3961 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     4021 gcgcgcggtg tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca
     4081 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     4141 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc
     4201 tgaattaggc gcgcctattt ctgaattcaa cacattgcgg acgtttttaa tgtactgaat
     4261 taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta
     4321 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
     4381 ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca
     4441 cattattatg gagaaactcg agcttgtcga tcgactctag ctagaggatc gatccgaacc
     4501 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     4561 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     4621 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     4681 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     4741 gccatgtgtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag
     4801 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     4861 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     4921 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     4981 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     5041 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     5101 ccacgatagc cgcgctgcct cgtcctggag ttcattcagg gcaccggaca ggtcggtctt
     5161 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     5221 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     5281 tgcgtgcaat ccatcttgtt caatccccat ggtcgatcga cagatctgcg aaagctcgag
     5341 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
     5401 cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
     5461 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     5521 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     5581 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
     5641 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
     5701 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
     5761 ttcttggagt agacgagagt gtcgtgctcc accatgttca catcaatcca cttgctttga
     5821 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     5881 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     5941 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     6001 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     6061 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     6121 gttggcaagc tgctctagcc aatacgcaaa ccgaattcag aaataaattc agcctaattc
     6181 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
     6241 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
     6301 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
     6361 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
     6421 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
     6481 tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt
     6541 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     6601 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     6661 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     6721 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     6781 taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     6841 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     6901 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     6961 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     7021 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     7081 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     7141 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     7201 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     7261 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     7321 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     7381 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     7441 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     7501 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     7561 ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg
     7621 gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
     7681 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
     7741 atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7801 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7861 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7921 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7981 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8041 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8101 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8161 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8221 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8281 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8341 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8401 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8461 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8521 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8581 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     8641 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     8701 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     8761 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     8821 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     8881 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg
     8941 gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac
     9001 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt
     9061 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa
     9121 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca
     9181 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa
     9241 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt
     9301 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg
     9361 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct
     9421 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac
     9481 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc
     9541 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg
     9601 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga
     9661 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc
     9721 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc
     9781 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg
     9841 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt
     9901 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt
     9961 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg
    10021 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca
    10081 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc
    10141 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc
    10201 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc
    10261 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt
    10321 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc
    10381 cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt
    10441 tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt
    10501 tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc
    10561 gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta
    10621 gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca
    10681 tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg
    10741 tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac
    10801 ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga
    10861 tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc
    10921 aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga
    10981 ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt
    11041 gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat
    11101 gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg
    11161 caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg
    11221 gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat
    11281 cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc
    11341 gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag
    11401 ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac
    11461 agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac
    11521 gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg
    11581 cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa
    11641 ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac
    11701 ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg
    11761 tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt
    11821 cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca
    11881 cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag
    11941 ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg
    12001 ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc
    12061 cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg
    12121 tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca
    12181 ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg
    12241 ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc
    12301 tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc
    12361 agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc
    12421 gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct
    12481 agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg
    12541 gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg
    12601 gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac
    12661 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
    12721 ct
//
