LOCUS       sequence-121739-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAPT110 encoding rbsH-hrpR-ter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Wang B, Kitney RI, Joly N, Buck M
  TITLE     Engineering modular and orthogonal genetic logic gates for robust 
            digital-like synthetic biology.
  JOURNAL   Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
  PUBMED    22009040
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-10-18"; volume: "2:508. doi"; pages: " 
            10.1038/ncomms1516"
COMMENT     Sequence Label: pBW115lac-hrpR
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..92)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(133..144)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gagccagtgt gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc
       61 cagtctttcg actgagcctt tcgttttatt tgatgcctgg ttattagacc ccggccgtcg
      121 cagcgatcgg cgctgcaact cccagttcct tcatgcggtg atacagggtg cgtcttggca
      181 actccaactc ctgaagcacc gcgtcgaaat tgtgcctgtg ccgcttcaag gcatcctgga
      241 tgagcatttt ctcgatgatg cgcatttgcg tgcgcagccc cgtggcaggg tcaagcgctt
      301 ccacagggtc ggcgcccagc aaggggaagc cgagtacgaa gcgcttggcc gcagacttca
      361 attcgcggat gttgcccggc cagtcgtggc tgagcagcag ctgcacacgc ccgctgtcca
      421 gcgcaggagc gggacgtccg aactcggcag cgataccctg ggtgaactgg tcgaacaatg
      481 gcaggatctg ttcacgacgt ttgcgcaagg ctggcaagtg aagcgtcagc acgttgagcc
      541 gaaaaaacag gtcgcgacgg aaaagtcctt gttccaccag ttcatccagt ggccgctggg
      601 ccgaggcaat gatccgcaga tccaccggga taaattcggt cgagcccaga cgctcgatac
      661 ctcgactctc caacacacgc agcagtttgg cttgcaggct caacggcatg ctgtcgattt
      721 catccaggta caaggtgcca ccactggagg cctctatgta gccctcgcga gcccgg
//
