LOCUS       sequence-12174-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Carlson HJ, Cotton DW, Campbell RE
  TITLE     Circularly permuted monomeric red fluorescent proteins with new 
            termini in the beta-sheet.
  JOURNAL   Protein Sci. 2010 Aug;19(8):1490-9. doi: 10.1002/pro.428.
  PUBMED    20521333
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro"; 
            journalName: "Protein Sci"; date: "2010-08"; volume: "19"; issue: 
            "8"; pages: "1490-9"
COMMENT     Sequence Label: cp201
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             22..54
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(58..66,67..81)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(380..398)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     648..667
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat
       61 ctgtacgacg atgacgataa ggatccgagc tcgagcatgt ccagctccgg ggcactgggc
      121 ggtgtgacct cacacaacga ggactacacc atcgtggaac agtacgaacg cgccgaggcc
      181 cgccactcca ccggcggcat ggacgagctg tacaagggtg gcactggcgg ttccatggtg
      241 agcaagggcg tggaggataa catggccttc atcaaggagt tcatgcgctt caaggtgcac
      301 atggagggct ccgtcaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      361 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
      421 tgggacatcc tgtcccctca gctcatgtac ggctccaagg cctacgtgaa gcaccccgcc
      481 gacatccccg actactggaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      541 aacttcgagg acggaggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
      601 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
      661 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgctctg
      721 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
      781 aagaccacct acaaggccaa gaagcctgtg cagctgcccg gcgcctacaa cgtcgacatc
      841 aagttgacag gcggtgacca actgacagaa gagtga
//
