LOCUS       sequence-121740-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying 
            BBa_F2620 (PTet-rbs34-luxR-ter)).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Wang B, Kitney RI, Joly N, Buck M
  TITLE     Engineering modular and orthogonal genetic logic gates for robust 
            digital-like synthetic biology.
  JOURNAL   Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
  PUBMED    22009040
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-10-18"; volume: "2:508. doi"; pages: " 
            10.1038/ncomms1516"
COMMENT     Sequence Label: pBW313lux-hrpR
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    14..35
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    36..54
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    61..79
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             98..109
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aggatgattt ctggaattcg cggccgcttc tagagtccct atcagtgata gagattgaca
       61 tccctatcag tgatagagat actgagcact actagagaaa gaggagaaat actagatgaa
      121 aaacataaat gccgacgaca catacagaat aattaataaa attaaagctt gtagaagcaa
      181 taatgatatt aatcaatgct tatctgatat gactaaaatg gtacattgtg aatattattt
      241 actcgcgatc atttatcctc attctatggt taaatctgat atttcaatcc tagataatta
      301 ccctaaaaaa tggaggcaat attatgatga cgctaattta ataaaatatg atcctatagt
      361 agattattct aactccaatc attcaccaat taattggaat atatttgaaa acaatgctgt
      421 aaataaaaaa tctccaaatg taattaaaga agcgaaaaca tcaggtctta tcactgggtt
      481 tagtttccct attcatacgg ctaacaatgg cttcggaatg cttagttttg cacattcaga
      541 aaaagacaac tatatagata gtttattttt acatgcgtgt atgaacatac cattaattgt
      601 tccttctcta gttgataatt atcgaaaaat aaatatagca aataataaat caaacaacga
      661 tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga
      721 tatttcaaaa atattaggtt gcagtgagcg tactgtcact ttccatttaa ccaatgcgca
      781 aatgaaactc aatacaacaa accgctgcca aagtatttct aaagcaattt taacaggagc
      841 aattgattgc ccatacttta aaaattaata acactgatag tgctagtgta gatcactact
      901 agagccaggc atc
//
