LOCUS       sequence-121773-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA for csr-1 locus in N. crassa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Matsu-Ura T, Baek M, Kwon J, Hong C
  TITLE     Efficient gene editing in Neurospora crassa with CRISPR technology.
  JOURNAL   Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 
            10.1186/s40694-015-0015-1. eCollection 2015.
  PUBMED    28955455
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2015-07-15"; volume: "2:4. doi"; pages: " 
            10.1186/s40694-015-0015-1. eCollection 2015"
COMMENT     Sequence Label: p426-SNR52p-gRNA.csr-1.Y-SUP4t
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..37
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        19..37
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        55..323
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     misc_RNA        344..418
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      423..442
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     terminator      448..695
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(713..732)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(714..732)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(742..759)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(742..758)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agcttctttg
       61 aaaagataat gtatgattat gctttcactc atatttatac agaaacttga tgttttcttt
      121 cgagtatata caaggtgatt acatgtacgt ttgaagtaca actctagatt ttgtagtgcc
      181 ctcttgggct agcggtaaag gtgcgcattt tttcacaccc tacaatgttc tgttcaaaag
      241 attttggtca aacgctgtag aagtgaaagt tggtgcgcat gtttcggcgt tcgaaacttc
      301 tccgcagtga aagataaatg atcgagtggg agggtcccgt cctgttttag agctagaaat
      361 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtggt
      421 gctttttttg ttttttatgt cttcgagtca tgtaattagt tatgtcacgc ttacgttcac
      481 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
      541 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
      601 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
      661 agaaggtttt gggacgctcg aaggctttaa tttgcggccg gtacccaatt cgccctatag
      721 tgagtcgtat tacgcgcgct cactggccgt cgttttaca
//
