LOCUS       sequence-121778-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NPY-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Barg S, Knowles MK, Chen X, Midorikawa M, Almers W
  TITLE     Syntaxin clusters assemble reversibly at sites of secretory granules
            in live cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Nov 30;107(48):20804-9. doi: 
            10.1073/pnas.1014823107. Epub 2010 Nov 12.
  PUBMED    21076041
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1014823107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-11-30- 30"; volume: "107"; issue: "48"; pages: "20804-9"
COMMENT     Sequence Label: NPY-mCherry
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      404..413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(555..573)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     823..842
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttagcca ccatgctagg taacaagcga ctggggctgt ccggactgac
      121 cctcgccctg tccctgctcg tgtgcctggg tgcgctggcc gaggcgtacc cctccaagcc
      181 ggacaacccg ggcgaggacg caccagcgga ggacatggcc agatactact cggcgctgcg
      241 acactacatc aacctcatca ccaggcagag atatggaaaa cgatccagcc cagagacact
      301 gatttcagac ctcttgatga gagaaagcac agaaaatgtt cccagaactc ggcttgaaga
      361 ccctgcaatg tggacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa
      421 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
      481 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      541 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
      601 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
      661 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      721 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
      781 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
      841 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
      901 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
      961 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     1021 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     1081 ccgccactcc
//
