LOCUS       sequence-12178-e        7668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Yan Q, Bartz S, Mao M, Li L, Kaelin WG
  TITLE     The hypoxia-inducible factor 2alpha N-terminal and C-terminal 
            transactivation domains cooperate to promote renal tumorigenesis in 
            vivo.
  JOURNAL   Mol Cell Biol. 2007 Mar . 27(6):2092-102.
  PUBMED    17220275
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Mar . 27(6):2092-102."
COMMENT     Sequence Label: HA-HIF2-alpha bHLH* P405A/P531A 
            Delta-820-870-pBabe-Puro
FEATURES             Location/Qualifiers
     source          1..7668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1318..1334
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1379..1405
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3907..3927)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3912..4241
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4092..4227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4251..4850
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4251..4270)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4747..4767
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4949..5542
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5743..5760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5914..6502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5994..6013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6680..7471,7472..7540))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7303..7322
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7541..7645)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
     1321 tatccagccc tcactccttc tctaggcgcc ggccatcgag actaagctta ccatggccta
     1381 cccctatgat gttcccgatt atgctggatc cgccaccatg acagctgaca aggagaagaa
     1441 aaggagtagc tcggagagga ggaaggagaa gtcccgggat gctgcggctt gcgctgctag
     1501 cgctgagacg gaggtgttct atgagctggc ccatgagctg cctctgcccc acagtgtgag
     1561 ctcccatctg gacaaggcct ccatcatgcg actggcaatc agcttcctgc gaacacacaa
     1621 gctcctctcc tcagtttgct ctgaaaacga gtccgaagcc gaagctgacc agcagatgga
     1681 caacttgtac ctgaaagcct tggagggttt cattgccgtg gtgacccaag atggcgacat
     1741 gatctttctg tcagaaaaca tcagcaagtt catgggactt acacaggtgg agctaacagg
     1801 acatagtatc tttgacttca ctcatccctg cgaccatgag gagattcgtg agaacctgag
     1861 tctcaaaaat ggctctggtt ttgggaaaaa aagcaaagac atgtccacag agcgggactt
     1921 cttcatgagg atgaagtgca cggtcaccaa cagaggccgt actgtcaacc tcaagtcagc
     1981 cacctggaag gtcttgcact gcacgggcca ggtgaaagtc tacaacaact gccctcctca
     2041 caatagtctg tgtggctaca aggagcccct gctgtcctgc ctcatcatca tgtgtgaacc
     2101 aatccagcac ccatcccaca tggacatccc cctggatagc aagaccttcc tgagccgcca
     2161 cagcatggac atgaagttca cctactgtga tgacagaatc acagaactga ttggttacca
     2221 ccctgaggag ctgcttggcc gctcagccta tgaattctac catgcgctag actccgagaa
     2281 catgaccaag agtcaccaga acttgtgcac caagggtcag gtagtaagtg gccagtaccg
     2341 gatgctcgca aagcatgggg gctacgtgtg gctggagacc caggggacgg tcatctacaa
     2401 ccctcgcaac ctgcagcccc agtgcatcat gtgtgtcaac tacgtcctga gtgagattga
     2461 gaagaatgac gtggtgttct ccatggacca gactgaatcc ctgttcaagc cccacctgat
     2521 ggccatgaac agcatctttg atagcagtgg caagggggct gtgtctgaga agagtaactt
     2581 cctattcacc aagctaaagg aggagcccga ggagctggcc cagctggctg ctaccccagg
     2641 agacgccatc atctctctgg atttcgggaa tcagaacttc gaggagtcct cagcctatgg
     2701 caaggccatc ctgcccccga gccagccatg ggccacggag ttgaggagcc acagcaccca
     2761 gagcgaggct gggagcctgc ctgccttcac cgtgccccag gcagctgccc cgggcagcac
     2821 cacccccagt gccaccagca gcagcagcag ctgctccacg cccaatagcc ctgaagacta
     2881 ttacacatct ttggataacg acctgaagat tgaagtgatt gagaagctct tcgccatgga
     2941 cacagaggcc aaggaccaat gcagtaccca gacggatttc aatgagctgg acttggagac
     3001 actggcagct tatatcccca tggacgggga agacttccag ctaagcccca tctgccccga
     3061 ggagcggctc ttggcggaga acccacagtc caccccccag cactgcttca gtgccatgac
     3121 aaacatcttc cagccactgg cccctgtagc cccgcacagt cccttcctcc tggacaagtt
     3181 tcagcagcag ctggagagca agaagacaga gcccgagcac cggcccatgt cctccatctt
     3241 ctttgatgcc ggaagcaaag catccctgcc accgtgctgt ggccaggcca gcacccctct
     3301 ctcttccatg gggggcagat ccaataccca gtggccccca gatccaccat tacattttgg
     3361 gcccacaaag tgggccgtcg gggatcagcg cacagagttc ttgggagcag cgccgttggg
     3421 gccccctgtc tctccacccc atgtctccac cttcaagaca aggtctgcaa agggttttgg
     3481 ggctcgaggc ccagacgtgc tgagtccggc catggtagcc ctctccaaca agctgaagct
     3541 gaagcgacag ctggagtatg aagagcaagc cttccaggac ctgagcgggg gggacccacc
     3601 tggtggcagc acctcacatt tgatgtggaa acggatgaag aacctcaggg gtgggagctg
     3661 ccctttgatg ccggacaagc cactgagcgc aaatgtaccc aatgataagt tcacccaaaa
     3721 ccccatgagg ggcctgggcc atcccctgag acatctgccg ctgccacagc ctccatctgc
     3781 catcagtccc ggggagaaca gcaagagcag gttcccccca cagtgctacg ccacccagta
     3841 ccaggactac agcctgtcgt cagcccacaa ggtgtgacaa ttcgccagca cagtggtcga
     3901 ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3961 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4021 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4081 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4141 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4201 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttacc atgaccgagt
     4261 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     4321 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     4381 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     4441 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     4501 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     4561 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     4621 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     4681 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     4741 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagt
     4801 gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     4861 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgata aaataaaaga
     4921 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     4981 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt
     5041 tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     5101 taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa
     5161 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
     5221 agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
     5281 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
     5341 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg
     5401 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
     5461 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
     5521 ccgtcagcgg gggtctttca tttccgactt gtggtctcgc tgccttggga gggtctcctc
     5581 tgagtgattg actacccgtc agcgggggtc ttcacatgca gcatgtatca aaattaattt
     5641 ggtttttttt cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac
     5701 gcgcggggag aggcggtttg cgtattggcg ctcttccgct tcctcgctca ctgactcgct
     5761 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5821 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5881 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5941 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6001 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6061 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6121 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6181 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6241 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6301 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     6361 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6421 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6481 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6541 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6601 ctagatcctt ttaaattaaa aatgaagttt gcggccgcaa atcaatctaa agtatatatg
     6661 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6721 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6781 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6841 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6901 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6961 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7021 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7081 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7141 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7201 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7261 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     7321 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7381 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7441 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7501 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7561 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7621 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
