LOCUS       sequence-121784-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with C-term 
            8xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G02
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    99..223
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(270..575)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(372..391)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tttattttat tttattttat tttattttat tttatttaac catgacagta atgtataaag
       61 tctgtaaaga cattaaacac gtaagtgaaa ccatgggcac aagtttgtac aaaaaagctg
      121 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
      181 agactacata atactgtaaa acacaacata tccagtcact atggggcccg tcgacctgca
      241 gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg
      301 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc
      361 ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc
      421 gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc
      481 atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg
      541 ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc
      601 aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc
      661 tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc
      721 ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact
      781 gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat
      841 atattcttat accgcaaaaa atcagcgcgc aaatacgcat a
//
