LOCUS       sequence-121787-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with N-term 
            8xHis sfGFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G09
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             108..131
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(168..196)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttattttat tttattttat tttattttat tttatttaac catgacagta atgtataaag
       61 tctgtaaaga cattaaacac gtaagtgaaa ccatgggcac ctctggtcac caccatcacc
      121 accatcacca cggcacaatg agcaaaggag aagaactttt cactggagtt gtcccaattc
      181 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtccgtgga gagggtgaag
      241 gtgatgctac aaacggaaaa ctcaccctta aatttatttg cactactgga aaactacctg
      301 ttccgtggcc aacacttgtc actactctga cctatggtgt tcaatgcttt tcccgttatc
      361 cggatcacat gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      421 aacgcactat atctttcaaa gatgacggga cctacaagac gcgtgctgaa gtcaagtttg
      481 aaggtgatac ccttgttaat cgtatcgagt taaagggtat tgattttaaa gaagatggaa
      541 acattcttgg acacaaactc gagtacaact ttaactcaca caatgtatac atcacggcag
      601 acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacgtt gaagatggtt
      661 ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      721 taccagacaa ccattacctg tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc
      781 gtgaccacat ggtccttctt gagt
//
