LOCUS       sequence-121792-         440 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite Gateway destination vector to create transgenes for 
            phiC31-mediated transgenesis in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI
  TITLE     Site-directed zebrafish transgenesis into single landing sites with 
            the phiC31 integrase system.
  JOURNAL   Dev Dyn. 2013 Aug;242(8):949-63. doi: 10.1002/dvdy.23989. Epub 2013 
            Jul 3.
  PUBMED    23723152
REFERENCE   2  (bases 1 to 440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.23989"; journalName: "Dev Dyn"; date: "2013-08"; 
            volume: "242"; issue: "8"; pages: "949-63"
COMMENT     Sequence Label: pDestattB (pCM268)
FEATURES             Location/Qualifiers
     source          1..440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..80
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     64..80
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    100..223
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(366..385)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gcactggatc tagcggatcc gggaattggg aattcgttaa cagatctgcg gccgcggctc
       61 gagcaggaaa cagctatgac catgattacg ccaagctatc aactttgtat agaaaagttg
      121 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
      181 agactacata atactgtaaa acacaacata tccagtcact atggtcgacc tgcagactgg
      241 ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg
      301 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca
      361 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa
      421 agttcacggg ggactttatc
//
