LOCUS       sequence-121798-       11477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB215
FEATURES             Location/Qualifiers
     source          1..11477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2050..2052,2053..2055,2056..2766)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2095..2116)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2356..2375)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2703..2724
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2773..2793
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2818..3070
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3104..3120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3104..3120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3117..3139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3128..3144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3152..3158,3159..3176,3177..3182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3197..3218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3303..3475
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3532..4065)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4128..4804)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4171..4191)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    4999..5023
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5544..6335
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6581..7169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7070..7089
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7323..7340
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7355..7495
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7421..7440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7581..7603
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7839..8033
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8099..9172)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9601..10230)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2101 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2161 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2221 tggcccaccc tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac
     2281 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2341 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2401 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2461 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2521 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2581 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2641 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2701 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2761 tacaagttgc agcctaagaa gaagagaaag gttggaggat agagctcgaa tttccccgat
     2821 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     2881 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     2941 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     3001 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     3061 ttactagatc gggaattggt tccggaacca attcgtaatc atggtcatag ctgtttcctg
     3121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3181 aagcctgggg tgcctaatga gtgagctaac tcacattagg ctgaattagg cgcgcctatt
     3241 tctgaattca acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg
     3301 gggatctgga ttttagtact ggattttggt tttaggaatt agattttatt gatagaagta
     3361 ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc
     3421 tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga
     3481 gcttgtcgat cgactctagg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
     3541 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
     3601 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
     3661 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
     3721 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
     3781 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
     3841 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
     3901 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
     3961 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
     4021 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt accatggtcg atcgacagat
     4081 ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc
     4141 tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt
     4201 gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt
     4261 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     4321 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
     4381 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     4441 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
     4501 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttcacatca
     4561 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     4621 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     4681 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     4741 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     4801 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     4861 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgaa ttcagaaata
     4921 aattcagcct aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     4981 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     5041 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     5101 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     5161 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     5221 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt
     5281 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     5341 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     5401 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     5461 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     5521 gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac
     5581 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     5641 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     5701 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     5761 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     5821 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     5881 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     5941 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     6001 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     6061 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     6121 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     6181 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     6241 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     6301 tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc
     6361 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
     6421 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
     6481 tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6541 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6601 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6661 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6721 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6781 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6841 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6901 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6961 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7021 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7081 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7141 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7201 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7261 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7321 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     7381 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     7441 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     7501 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     7561 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     7621 gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg
     7681 cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg
     7741 ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg
     7801 ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt
     7861 taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta
     7921 tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg
     7981 ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga
     8041 gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt
     8101 aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc
     8161 cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct
     8221 tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg
     8281 tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa
     8341 aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt
     8401 tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg
     8461 gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata
     8521 ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt
     8581 ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc
     8641 agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc
     8701 cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt
     8761 aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa
     8821 gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca
     8881 cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat
     8941 ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt
     9001 gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt
     9061 ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat
     9121 cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc
     9181 ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc
     9241 tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc
     9301 ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc
     9361 tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt
     9421 ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc
     9481 gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc
     9541 tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac
     9601 tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc
     9661 ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg
     9721 ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc
     9781 accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc
     9841 atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc
     9901 gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc
     9961 caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt
    10021 cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg
    10081 cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc
    10141 cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc
    10201 tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact
    10261 catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac
    10321 ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc
    10381 ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc
    10441 ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac
    10501 ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg
    10561 cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg
    10621 cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca
    10681 cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg
    10741 cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct
    10801 tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa
    10861 tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct
    10921 ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt
    10981 cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta
    11041 ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg
    11101 aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt
    11161 cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt
    11221 aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa
    11281 aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt
    11341 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
    11401 gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg
    11461 gacgaacgga taaacct
//
