LOCUS       sequence-121806-       11250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 11250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB117
FEATURES             Location/Qualifiers
     source          1..11250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2110..2362
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2396..2412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2396..2412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2409..2431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2420..2436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2444..2450,2451..2468,2469..2474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2489..2510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    2595..2769
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2810..3835)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(3902..4577)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    4772..4796
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5317..6108
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6354..6942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6843..6862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7096..7113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7128..7268
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7194..7213)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7354..7376
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7612..7806
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7872..8945)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9374..10003)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagct tggatttgga tttggagttg cgcttgggat tcgcctaggc ttagagctcg
     2101 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     2161 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     2221 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     2281 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     2341 gtgtcatcta tgttactaga tcgggaattg gttccggaac caattcgtaa tcatggtcat
     2401 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     2461 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta ggctgaatta
     2521 ggcgcgccta tttctgaatt caacacattg cggacgtttt taatgtactg aattaacgcc
     2581 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
     2641 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
     2701 catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc acacattatt
     2761 atggagaaac tcgagcttgt cgatcgacag atcccggtcg gcatctactc tatttctttg
     2821 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
     2881 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
     2941 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
     3001 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg
     3061 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
     3121 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
     3181 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
     3241 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
     3301 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
     3361 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
     3421 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
     3481 tagcctccgc gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
     3541 tgacaccctg tgaacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt
     3601 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
     3661 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
     3721 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
     3781 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc
     3841 attgcccccc ggatctgcga aagctcgaga gagatagatt tgtagagaga gactggtgat
     3901 ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggt cttgcgaagg
     3961 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
     4021 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     4081 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
     4141 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
     4201 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
     4261 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
     4321 catgttcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
     4381 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga
     4441 tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc
     4501 ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
     4561 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg
     4621 agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa tacgcaaacc
     4681 gaattcagaa ataaattcag cctaattcgg cgttaattca gtacattaaa aacgtccgca
     4741 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
     4801 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     4861 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     4921 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     4981 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg
     5041 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     5101 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     5161 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     5221 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     5281 cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt gatcgccgaa
     5341 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     5401 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     5461 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     5521 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     5581 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     5641 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     5701 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     5761 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     5821 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     5881 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     5941 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     6001 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     6061 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctagctaga
     6121 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     6181 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     6241 gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt
     6301 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6361 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6421 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6481 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     6541 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6601 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6661 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6721 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6781 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6841 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6901 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6961 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7021 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7081 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     7141 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     7201 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     7261 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     7321 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     7381 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt
     7441 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
     7501 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
     7561 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag
     7621 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
     7681 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
     7741 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
     7801 tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
     7861 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
     7921 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
     7981 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
     8041 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
     8101 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
     8161 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc
     8221 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct
     8281 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca
     8341 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca
     8401 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc
     8461 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc
     8521 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg
     8581 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga
     8641 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc
     8701 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg
     8761 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca
     8821 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc
     8881 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg
     8941 ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc
     9001 agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg
     9061 tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca
     9121 tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta
     9181 atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac
     9241 tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg
     9301 ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct
     9361 ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc
     9421 gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg
     9481 ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg
     9541 cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg
     9601 cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg
     9661 tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga
     9721 tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc
     9781 ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg
     9841 gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta
     9901 acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct
     9961 tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat
    10021 ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc
    10081 aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg
    10141 ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga
    10201 gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc
    10261 ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg
    10321 cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca
    10381 ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg
    10441 gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg
    10501 atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc
    10561 acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc
    10621 tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg
    10681 tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca
    10741 atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg
    10801 tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg
    10861 gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg
    10921 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
    10981 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
    11041 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
    11101 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
    11161 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
    11221 gcacatacaa atggacgaac ggataaacct
//
