LOCUS       sequence-121808-       12203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12203)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB317
FEATURES             Location/Qualifiers
     source          1..12203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2110..2362
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2396..2412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2396..2412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2409..2431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2420..2436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2444..2450,2451..2468,2469..2474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2489..2510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    2595..2770
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(4854..5530)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4897..4917)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5725..5749
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6270..7061
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7307..7895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7796..7815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8049..8066
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8081..8221
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8147..8166)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8307..8329
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8565..8759
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8825..9898)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10327..10956)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagct tggatttgga tttggagttg cgcttgggat tcgcctaggc ttagagctcg
     2101 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     2161 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     2221 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     2281 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     2341 gtgtcatcta tgttactaga tcgggaattg gttccggaac caattcgtaa tcatggtcat
     2401 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     2461 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta ggctgaatta
     2521 ggcgcgccta tttctgaatt caacacattg cggacgtttt taatgtactg aattaacgcc
     2581 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
     2641 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
     2701 catgagcgaa accctatagg aaccctaaat tcccttatct gggaactact cacacattat
     2761 tatggagaaa ctcgaagtta gtatatagtc cttccatcac cctctgtgat aatctcctta
     2821 aaacttgctc ccccaggaat cataggcaga acgtgctcct ggtggggaac tatcacatcc
     2881 agtaaataag gaccgggaga atctagcata gtttgaattg ctgctcttag ttcatccttc
     2941 ttcaccacac gcgcatttgg caccttgaaa ccgtctgcaa ctgtacagaa gtcggggtag
     3001 atctgctcct cgtgatctgg atcacctaag taagtatgtg ctctgttggc cttgtagaat
     3061 ctatcctcca attgtacgac cattcctaga tgttggttgt ttaagatcat gatcttcacg
     3121 ggtaacttct ccacaaaaat tgtagccaac tcctgtacgt tcatcacaaa gctaccatcg
     3181 ccatcgatgt cgatcacagg aatatctggt tgagttgcgg cagcccccaa agcagaagga
     3241 agtccgaatc ccatggctcc aagacctcca gatgtcaacc aacgccgcgg ttcgttgtac
     3301 ttgaaccact gggctgccca catttgatgt tggccaacac cagtagatat gatggcattt
     3361 ccaccagtga gttcgtacag cgtctgtatg gcagattgag gagcaatggc atcctcgaat
     3421 acgggaaact tcattggcca tttctgcttt tgttcttcta gttctgccac ccagttggag
     3481 aagtcgaaag tcctcttttg actcttcaag agggcattga tttcacttag cgcaggtttc
     3541 acatcagcac acatggacac atggggctgt ttgttctttc caatttcagc agggtcaata
     3601 tcgatatgaa cgatgctgga cctgctagca aaagtctcca gtttaccagt cactcgatcg
     3661 tcgaaacgca ctccaaatgc caacaacaga tctgcttggt ccacggcgta gttggcgtaa
     3721 acagttccat gcattccaag cattttcaaa cttaatggat cagactcggg atacgctccc
     3781 aaacccatta aagtcgatgt gataggaatt ccggtaagct cgacaaattc cctcaactct
     3841 tcgctggcgt gattgcaacc tcctccagaa tacactacag gcttcttaga actagaaatg
     3901 agtcttataa tctgctcgat taacgttggt tccggtggcc gaggcagtcg ttgtaagtag
     3961 cccttcagtt gcattggctg agaccaatct ggaatagcca tttgctgttg cacgtccttg
     4021 ggaatatcta ccaagactgg gcccggccta cctgaagctg ccaaaaagaa agcctccctg
     4081 attacgcgag ggatgtcatc aatgttcata acaaggtaat tgtgcttggt gatcgatctc
     4141 gtaacctcca caattggagt ttcttgaaaa gcatcggtac caatcatcct actggatact
     4201 tgacctgtaa tggccactag tggcacactg tccaagaggg catcagcgag cccggtaaca
     4261 agattggtcg ctccaggacc ggatgtggct atacaaaccc ctactcgtcc agacgattta
     4321 gcataacctt cagcagcgaa aatctccccc tgctcgtggc gacagagaat gttgcggatg
     4381 ctgctggaac gtgtaagagc ctggtgaatc tccatagacg ctccgccggg ataagcgaac
     4441 accgttctaa tcccttctcg ctcaagagcc tccaccaaaa tgtcggctcc tttgcggggc
     4501 tcgtcatcct tgtagcgaga ctgaaagtcg gacggggttg gtgcaacgag tgacttcccg
     4561 ctgctaagcc gtggtccact cacatcgact gcagactgga tcttctcgct aacagcttgg
     4621 gctttcactt ccagtgaacg ctcagtcttc cgagaggtgt tgtatgcatg tcccctcaga
     4681 gcggctcccg agaagaaacc actcctcacc tttagctctt tagctccctt gcaacaagac
     4741 cttgccgatg gctgcacctt ctccaggctc gctatgctca aaggcacagc tgcgcatcca
     4801 ctaattgacg ccatgctcga gagagataga tttgtagaga gagactggtg atttcagcgt
     4861 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
     4921 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
     4981 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     5041 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatt atggcatttg
     5101 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
     5161 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
     5221 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttc
     5281 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     5341 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     5401 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     5461 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     5521 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     5581 gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa accgaattca
     5641 gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
     5701 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     5761 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     5821 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     5881 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     5941 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa
     6001 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     6061 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     6121 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     6181 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     6241 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     6301 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     6361 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     6421 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     6481 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     6541 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     6601 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     6661 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     6721 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     6781 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     6841 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     6901 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     6961 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     7021 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     7081 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     7141 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     7201 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
     7261 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7321 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7381 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7441 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7501 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7561 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7621 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7681 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7741 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7801 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7861 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7921 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7981 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8041 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     8101 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     8161 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     8221 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     8281 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     8341 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     8401 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     8461 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     8521 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     8581 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     8641 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     8701 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     8761 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     8821 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     8881 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     8941 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     9001 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     9061 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     9121 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     9181 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     9241 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     9301 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     9361 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     9421 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
     9481 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
     9541 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
     9601 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
     9661 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
     9721 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
     9781 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
     9841 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc
     9901 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct
     9961 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg
    10021 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
    10081 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
    10141 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
    10201 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
    10261 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
    10321 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
    10381 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
    10441 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
    10501 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
    10561 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
    10621 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
    10681 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
    10741 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
    10801 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
    10861 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
    10921 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
    10981 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
    11041 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
    11101 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
    11161 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
    11221 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
    11281 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
    11341 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
    11401 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
    11461 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
    11521 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
    11581 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
    11641 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
    11701 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
    11761 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
    11821 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
    11881 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
    11941 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
    12001 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
    12061 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
    12121 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
    12181 caaatggacg aacggataaa cct
//
