LOCUS       sequence-121811-       12503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB218
FEATURES             Location/Qualifiers
     source          1..12503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2302..2961
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2368..2387)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3487..3506
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3844..4096
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4130..4146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4130..4146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4143..4165)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4154..4170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4178..4184,4185..4202,4203..4208))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4223..4244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4329..4501
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4558..5091)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(5154..5830)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(5197..5217)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6025..6049
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6570..7361
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7607..8195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8096..8115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8349..8366
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8381..8521
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8447..8466)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8607..8629
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8865..9059
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9125..10198)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10627..11256)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcttggatt tggatttgga gttgcgcttg ggattcgcct aggcttagag ctcgaatttc
     3841 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     3901 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     3961 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     4021 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     4081 tctatgttac tagatcggga attggttccg gaaccaattc gtaatcatgg tcatagctgt
     4141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga attaggcgcg
     4261 cctatttctg aattcaacac attgcggacg tttttaatgt actgaattaa cgccgaatta
     4321 attcggggga tctggatttt agtactggat tttggtttta ggaattagat tttattgata
     4381 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
     4441 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
     4501 actcgagctt gtcgatcgac tctaggccgg gaagccgatc tcggcttgaa cgaattgtta
     4561 ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt
     4621 atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc
     4681 aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg
     4741 gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt
     4801 gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc
     4861 gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga
     4921 gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt
     4981 gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt
     5041 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctgcgtacca tggtcgatcg
     5101 acagatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
     5161 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
     5221 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
     5281 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     5341 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
     5401 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
     5461 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
     5521 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttc
     5581 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     5641 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     5701 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     5761 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     5821 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     5881 gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa accgaattca
     5941 gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
     6001 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     6061 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     6121 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     6181 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     6241 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa
     6301 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     6361 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     6421 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     6481 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     6541 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     6601 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     6661 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     6721 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     6781 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     6841 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     6901 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     6961 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     7021 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     7081 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     7141 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     7201 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     7261 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     7321 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     7381 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     7441 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     7501 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
     7561 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7621 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7681 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7741 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7801 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7861 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7921 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7981 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8041 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8101 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8161 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8221 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8281 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8341 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     8401 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     8461 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     8521 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     8581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     8641 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     8701 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     8761 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     8821 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     8881 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     8941 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     9001 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     9061 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     9121 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     9181 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     9241 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     9301 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     9361 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     9421 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     9481 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     9541 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     9601 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     9661 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     9721 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
     9781 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
     9841 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
     9901 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
     9961 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
    10021 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
    10081 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
    10141 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc
    10201 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct
    10261 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg
    10321 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
    10381 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
    10441 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
    10501 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
    10561 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
    10621 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
    10681 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
    10741 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
    10801 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
    10861 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
    10921 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
    10981 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
    11041 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
    11101 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
    11161 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
    11221 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
    11281 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
    11341 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
    11401 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
    11461 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
    11521 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
    11581 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
    11641 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
    11701 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
    11761 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
    11821 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
    11881 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
    11941 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
    12001 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
    12061 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
    12121 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
    12181 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
    12241 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
    12301 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
    12361 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
    12421 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
    12481 caaatggacg aacggataaa cct
//
