LOCUS       sequence-121816-       13059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13059)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB319
FEATURES             Location/Qualifiers
     source          1..13059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2966..3218
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3252..3268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3252..3268)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3265..3287)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3276..3292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3300..3306,3307..3324,3325..3330))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3345..3366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3451..3626
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(5710..6386)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    6581..6605
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7126..7917
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8163..8751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8652..8671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8905..8922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8937..9077
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9003..9022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9163..9185
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9421..9615
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9681..10754)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11183..11812)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcttgga tttggatttg gagttgcgct tgggattcgc
     2941 ctaggcttag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
     3001 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     3061 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     3121 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     3181 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattggttc cggaaccaat
     3241 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3301 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3361 acattaggct gaattaggcg cgcctatttc tgaattcaac acattgcgga cgtttttaat
     3421 gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt
     3481 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
     3541 tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaaattccc ttatctggga
     3601 actactcaca cattattatg gagaaactcg aagttagtat atagtccttc catcaccctc
     3661 tgtgataatc tccttaaaac ttgctccccc aggaatcata ggcagaacgt gctcctggtg
     3721 gggaactatc acatccagta aataaggacc gggagaatct agcatagttt gaattgctgc
     3781 tcttagttca tccttcttca ccacacgcgc atttggcacc ttgaaaccgt ctgcaactgt
     3841 acagaagtcg gggtagatct gctcctcgtg atctggatca cctaagtaag tatgtgctct
     3901 gttggccttg tagaatctat cctccaattg tacgaccatt cctagatgtt ggttgtttaa
     3961 gatcatgatc ttcacgggta acttctccac aaaaattgta gccaactcct gtacgttcat
     4021 cacaaagcta ccatcgccat cgatgtcgat cacaggaata tctggttgag ttgcggcagc
     4081 ccccaaagca gaaggaagtc cgaatcccat ggctccaaga cctccagatg tcaaccaacg
     4141 ccgcggttcg ttgtacttga accactgggc tgcccacatt tgatgttggc caacaccagt
     4201 agatatgatg gcatttccac cagtgagttc gtacagcgtc tgtatggcag attgaggagc
     4261 aatggcatcc tcgaatacgg gaaacttcat tggccatttc tgcttttgtt cttctagttc
     4321 tgccacccag ttggagaagt cgaaagtcct cttttgactc ttcaagaggg cattgatttc
     4381 acttagcgca ggtttcacat cagcacacat ggacacatgg ggctgtttgt tctttccaat
     4441 ttcagcaggg tcaatatcga tatgaacgat gctggacctg ctagcaaaag tctccagttt
     4501 accagtcact cgatcgtcga aacgcactcc aaatgccaac aacagatctg cttggtccac
     4561 ggcgtagttg gcgtaaacag ttccatgcat tccaagcatt ttcaaactta atggatcaga
     4621 ctcgggatac gctcccaaac ccattaaagt cgatgtgata ggaattccgg taagctcgac
     4681 aaattccctc aactcttcgc tggcgtgatt gcaacctcct ccagaataca ctacaggctt
     4741 cttagaacta gaaatgagtc ttataatctg ctcgattaac gttggttccg gtggccgagg
     4801 cagtcgttgt aagtagccct tcagttgcat tggctgagac caatctggaa tagccatttg
     4861 ctgttgcacg tccttgggaa tatctaccaa gactgggccc ggcctacctg aagctgccaa
     4921 aaagaaagcc tccctgatta cgcgagggat gtcatcaatg ttcataacaa ggtaattgtg
     4981 cttggtgatc gatctcgtaa cctccacaat tggagtttct tgaaaagcat cggtaccaat
     5041 catcctactg gatacttgac ctgtaatggc cactagtggc acactgtcca agagggcatc
     5101 agcgagcccg gtaacaagat tggtcgctcc aggaccggat gtggctatac aaacccctac
     5161 tcgtccagac gatttagcat aaccttcagc agcgaaaatc tccccctgct cgtggcgaca
     5221 gagaatgttg cggatgctgc tggaacgtgt aagagcctgg tgaatctcca tagacgctcc
     5281 gccgggataa gcgaacaccg ttctaatccc ttctcgctca agagcctcca ccaaaatgtc
     5341 ggctcctttg cggggctcgt catccttgta gcgagactga aagtcggacg gggttggtgc
     5401 aacgagtgac ttcccgctgc taagccgtgg tccactcaca tcgactgcag actggatctt
     5461 ctcgctaaca gcttgggctt tcacttccag tgaacgctca gtcttccgag aggtgttgta
     5521 tgcatgtccc ctcagagcgg ctcccgagaa gaaaccactc ctcaccttta gctctttagc
     5581 tcccttgcaa caagaccttg ccgatggctg caccttctcc aggctcgcta tgctcaaagg
     5641 cacagctgcg catccactaa ttgacgccat gctcgagaga gatagatttg tagagagaga
     5701 ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc
     5761 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
     5821 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
     5881 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
     5941 gcaattatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
     6001 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
     6061 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
     6121 gtgctccacc atgttcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
     6181 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
     6241 cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt
     6301 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
     6361 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga
     6421 tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat
     6481 acgcaaaccg aattcagaaa taaattcagc ctaattcggc gttaattcag tacattaaaa
     6541 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     6601 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     6661 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     6721 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     6781 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     6841 tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     6901 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     6961 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     7021 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     7081 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
     7141 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     7201 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     7261 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     7321 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     7381 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     7441 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     7501 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     7561 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     7621 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     7681 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     7741 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     7801 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     7861 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     7921 ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     7981 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     8041 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc
     8101 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8161 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8221 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8281 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     8341 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8401 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8461 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8521 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8581 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8641 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8701 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8761 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8821 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8881 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     8941 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     9001 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     9061 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     9121 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     9181 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
     9241 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
     9301 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
     9361 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
     9421 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
     9481 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
     9541 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
     9601 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
     9661 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
     9721 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
     9781 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
     9841 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
     9901 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
     9961 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
    10021 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
    10081 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
    10141 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
    10201 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
    10261 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
    10321 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
    10381 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
    10441 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
    10501 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
    10561 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
    10621 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
    10681 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
    10741 ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc
    10801 agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca
    10861 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc
    10921 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc
    10981 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc
    11041 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt
    11101 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc
    11161 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct
    11221 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg
    11281 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc
    11341 cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga
    11401 caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca
    11461 ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg
    11521 ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga
    11581 agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa
    11641 cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg
    11701 aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga
    11761 tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc
    11821 cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct
    11881 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
    11941 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
    12001 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
    12061 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
    12121 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
    12181 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
    12241 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
    12301 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
    12361 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
    12421 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
    12481 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
    12541 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
    12601 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
    12661 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
    12721 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
    12781 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
    12841 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
    12901 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg
    12961 actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt
    13021 ggttggcatg cacatacaaa tggacgaacg gataaacct
//
