LOCUS       sequence-121821-       11911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 11911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB420
FEATURES             Location/Qualifiers
     source          1..11911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2986..3238
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3272..3288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3272..3288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3285..3307)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3296..3312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3320..3326,3327..3344,3345..3350))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3365..3386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3471..3645
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3702..4499)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3813..3832)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4423..4442
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4562..5238)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4605..4625)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5433..5457
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5978..6769
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7015..7603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7504..7523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7757..7774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7789..7929
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7855..7874)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8015..8037
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8273..8467
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8533..9606)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10035..10664)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagct tggatttgga tttggagttg cgcttgggat tcgcctccgc cggaatggct
     2101 agtgaagctc gaaaaacaaa gaaaaaaatc aaagggattc agcaagccac tgcaggagtc
     2161 tcacaagaca cttcggaaaa tcctaacaaa acaatagttc ctgcagcatt accacagctc
     2221 acccctacct tggtgtcact gctggaggtg attgaacccg aggtgttgta tgcaggatat
     2281 gatagctctg ttccagattc agcatggaga attatgacca cactcaacat gttaggtggg
     2341 cgtcaagtga ttgcagcagt gaaatgggca aaggcgatac caggcttcag aaacttacac
     2401 ctggatgacc aaatgaccct gctacagtac tcatggatgt ttctcatggc atttgccctg
     2461 ggttggagat catacagaca atcaagtgga aacctgctct gctttgctcc tgatctgatt
     2521 attaatgagc agagaatgtc tctaccctgc atgtatgacc aatgtaaaca catgctgttt
     2581 gtctcctctg aattacaaag attgcaggta tcctatgaag agtatctctg tatgaaaacc
     2641 ttactgcttc tctcctcagt tcctaaggaa ggtctgaaga gccaagagtt atttgatgag
     2701 attcgaatga cttatatcaa agagctagga aaagccatcg tcaaaaggga agggaactcc
     2761 agtcagaact ggcaacggtt ttaccaactg acaaaacttc tggactccat gcatgaggtg
     2821 gttgagaatc tccttaccta ctgcttccag acatttttgg ataagaccat gagtattgaa
     2881 ttcccagaga tgttagctga aatcatcact aatcagatac caaaatattc aaatggaaat
     2941 atcaagaagc tgctgtttca tcaaaaatag agctcgaatt tccccgatcg ttcaaacatt
     3001 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     3061 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     3121 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     3181 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     3241 gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3301 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3361 cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc tgaattcaac
     3421 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
     3481 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
     3541 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
     3601 ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga gcttgtcgat
     3661 cgactctagc tagaggatcg atccgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     3721 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     3781 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     3841 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     3901 tccaccatga tattcggcaa gcaggcatcg ccatgtgtca cgacgagatc ctcgccgtcg
     3961 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     4021 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     4081 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     4141 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     4201 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     4261 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctggagt
     4321 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     4381 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     4441 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatccccatg
     4501 gtcgatcgac agatctgcga aagctcgaga gagatagatt tgtagagaga gactggtgat
     4561 ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
     4621 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     4681 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     4741 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
     4801 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
     4861 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
     4921 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
     4981 ccatgttcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
     5041 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
     5101 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
     5161 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
     5221 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
     5281 gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac
     5341 cgaattcaga aataaattca gcctaattcg gcgttaattc agtacattaa aaacgtccgc
     5401 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     5461 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     5521 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     5581 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     5641 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg
     5701 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     5761 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     5821 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     5881 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     5941 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
     6001 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     6061 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     6121 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     6181 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     6241 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     6301 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     6361 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     6421 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     6481 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     6541 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     6601 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     6661 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     6721 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     6781 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     6841 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     6901 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     6961 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7021 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7081 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7141 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     7201 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7261 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7321 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7381 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7441 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7501 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7561 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7621 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7681 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7741 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     7801 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     7861 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     7921 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7981 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     8041 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     8101 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     8161 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     8221 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     8281 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     8341 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     8401 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     8461 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     8521 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     8581 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     8641 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     8701 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     8761 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     8821 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     8881 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     8941 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     9001 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     9061 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     9121 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     9181 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     9241 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     9301 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     9361 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     9421 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     9481 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     9541 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     9601 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
     9661 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     9721 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     9781 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     9841 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     9901 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     9961 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
    10021 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
    10081 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
    10141 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
    10201 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
    10261 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
    10321 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
    10381 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
    10441 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
    10501 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
    10561 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
    10621 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
    10681 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
    10741 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
    10801 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
    10861 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
    10921 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
    10981 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
    11041 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
    11101 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
    11161 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
    11221 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
    11281 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
    11341 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
    11401 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
    11461 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
    11521 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
    11581 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
    11641 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
    11701 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
    11761 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
    11821 atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca
    11881 tgcacataca aatggacgaa cggataaacc t
//
