LOCUS       sequence-121824-       14813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14813)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 14813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB321
FEATURES             Location/Qualifiers
     source          1..14813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2302..2961
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2368..2387)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3487..3506
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4720..4972
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5006..5022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5006..5022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5019..5041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5030..5046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5054..5060,5061..5078,5079..5084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5099..5120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5205..5380
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(7464..8140)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(7507..7527)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    8335..8359
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8880..9671
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9917..10505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10406..10425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10659..10676
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10691..10831
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10757..10776)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10917..10939
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11175..11369
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11435..12508)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12937..13566)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcttggatt tggatttgga gttgcgcttg ggattcgcct ccgccggaat ggctagtgaa
     3841 gctcgaaaaa caaagaaaaa aatcaaaggg attcagcaag ccactgcagg agtctcacaa
     3901 gacacttcgg aaaatcctaa caaaacaata gttcctgcag cattaccaca gctcacccct
     3961 accttggtgt cactgctgga ggtgattgaa cccgaggtgt tgtatgcagg atatgatagc
     4021 tctgttccag attcagcatg gagaattatg accacactca acatgttagg tgggcgtcaa
     4081 gtgattgcag cagtgaaatg ggcaaaggcg ataccaggct tcagaaactt acacctggat
     4141 gaccaaatga ccctgctaca gtactcatgg atgtttctca tggcatttgc cctgggttgg
     4201 agatcataca gacaatcaag tggaaacctg ctctgctttg ctcctgatct gattattaat
     4261 gagcagagaa tgtctctacc ctgcatgtat gaccaatgta aacacatgct gtttgtctcc
     4321 tctgaattac aaagattgca ggtatcctat gaagagtatc tctgtatgaa aaccttactg
     4381 cttctctcct cagttcctaa ggaaggtctg aagagccaag agttatttga tgagattcga
     4441 atgacttata tcaaagagct aggaaaagcc atcgtcaaaa gggaagggaa ctccagtcag
     4501 aactggcaac ggttttacca actgacaaaa cttctggact ccatgcatga ggtggttgag
     4561 aatctcctta cctactgctt ccagacattt ttggataaga ccatgagtat tgaattccca
     4621 gagatgttag ctgaaatcat cactaatcag ataccaaaat attcaaatgg aaatatcaag
     4681 aagctgctgt ttcatcaaaa atagagctcg aatttccccg atcgttcaaa catttggcaa
     4741 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     4801 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     4861 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     4921 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     4981 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5041 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5101 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt caacacattg
     5161 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     5221 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     5281 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaaat
     5341 tcccttatct gggaactact cacacattat tatggagaaa ctcgaagtta gtatatagtc
     5401 cttccatcac cctctgtgat aatctcctta aaacttgctc ccccaggaat cataggcaga
     5461 acgtgctcct ggtggggaac tatcacatcc agtaaataag gaccgggaga atctagcata
     5521 gtttgaattg ctgctcttag ttcatccttc ttcaccacac gcgcatttgg caccttgaaa
     5581 ccgtctgcaa ctgtacagaa gtcggggtag atctgctcct cgtgatctgg atcacctaag
     5641 taagtatgtg ctctgttggc cttgtagaat ctatcctcca attgtacgac cattcctaga
     5701 tgttggttgt ttaagatcat gatcttcacg ggtaacttct ccacaaaaat tgtagccaac
     5761 tcctgtacgt tcatcacaaa gctaccatcg ccatcgatgt cgatcacagg aatatctggt
     5821 tgagttgcgg cagcccccaa agcagaagga agtccgaatc ccatggctcc aagacctcca
     5881 gatgtcaacc aacgccgcgg ttcgttgtac ttgaaccact gggctgccca catttgatgt
     5941 tggccaacac cagtagatat gatggcattt ccaccagtga gttcgtacag cgtctgtatg
     6001 gcagattgag gagcaatggc atcctcgaat acgggaaact tcattggcca tttctgcttt
     6061 tgttcttcta gttctgccac ccagttggag aagtcgaaag tcctcttttg actcttcaag
     6121 agggcattga tttcacttag cgcaggtttc acatcagcac acatggacac atggggctgt
     6181 ttgttctttc caatttcagc agggtcaata tcgatatgaa cgatgctgga cctgctagca
     6241 aaagtctcca gtttaccagt cactcgatcg tcgaaacgca ctccaaatgc caacaacaga
     6301 tctgcttggt ccacggcgta gttggcgtaa acagttccat gcattccaag cattttcaaa
     6361 cttaatggat cagactcggg atacgctccc aaacccatta aagtcgatgt gataggaatt
     6421 ccggtaagct cgacaaattc cctcaactct tcgctggcgt gattgcaacc tcctccagaa
     6481 tacactacag gcttcttaga actagaaatg agtcttataa tctgctcgat taacgttggt
     6541 tccggtggcc gaggcagtcg ttgtaagtag cccttcagtt gcattggctg agaccaatct
     6601 ggaatagcca tttgctgttg cacgtccttg ggaatatcta ccaagactgg gcccggccta
     6661 cctgaagctg ccaaaaagaa agcctccctg attacgcgag ggatgtcatc aatgttcata
     6721 acaaggtaat tgtgcttggt gatcgatctc gtaacctcca caattggagt ttcttgaaaa
     6781 gcatcggtac caatcatcct actggatact tgacctgtaa tggccactag tggcacactg
     6841 tccaagaggg catcagcgag cccggtaaca agattggtcg ctccaggacc ggatgtggct
     6901 atacaaaccc ctactcgtcc agacgattta gcataacctt cagcagcgaa aatctccccc
     6961 tgctcgtggc gacagagaat gttgcggatg ctgctggaac gtgtaagagc ctggtgaatc
     7021 tccatagacg ctccgccggg ataagcgaac accgttctaa tcccttctcg ctcaagagcc
     7081 tccaccaaaa tgtcggctcc tttgcggggc tcgtcatcct tgtagcgaga ctgaaagtcg
     7141 gacggggttg gtgcaacgag tgacttcccg ctgctaagcc gtggtccact cacatcgact
     7201 gcagactgga tcttctcgct aacagcttgg gctttcactt ccagtgaacg ctcagtcttc
     7261 cgagaggtgt tgtatgcatg tcccctcaga gcggctcccg agaagaaacc actcctcacc
     7321 tttagctctt tagctccctt gcaacaagac cttgccgatg gctgcacctt ctccaggctc
     7381 gctatgctca aaggcacagc tgcgcatcca ctaattgacg ccatgctcga gagagataga
     7441 tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat
     7501 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     7561 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     7621 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     7681 gcctttcctt tatcgcaatt atggcatttg taggtgccac cttccttttc tactgtcctt
     7741 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     7801 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     7861 tagacgagag tgtcgtgctc caccatgttc acatcaatcc acttgctttg aagacgtggt
     7921 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     7981 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     8041 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     8101 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     8161 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttggcaag
     8221 ctgctctagc caatacgcaa accgaattca gaaataaatt cagcctaatt cggcgttaat
     8281 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     8341 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     8401 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     8461 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     8521 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     8581 gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta
     8641 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     8701 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     8761 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     8821 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     8881 tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     8941 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     9001 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     9061 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     9121 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     9181 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     9241 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     9301 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     9361 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     9421 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     9481 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     9541 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     9601 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     9661 tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     9721 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
     9781 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat
     9841 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
    10081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10381 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10441 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10501 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10561 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10621 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10681 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
    10741 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
    10801 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
    10861 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
    10921 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
    10981 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
    11041 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
    11101 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
    11161 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
    11221 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
    11281 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
    11341 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
    11401 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
    11461 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
    11521 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
    11581 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
    11641 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
    11701 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
    11761 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
    11821 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
    11881 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
    11941 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
    12001 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
    12061 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
    12121 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
    12181 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
    12241 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
    12301 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
    12361 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
    12421 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
    12481 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
    12541 attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
    12601 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
    12661 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
    12721 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
    12781 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
    12841 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
    12901 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
    12961 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
    13021 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
    13081 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
    13141 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
    13201 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
    13261 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
    13321 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
    13381 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
    13441 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
    13501 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
    13561 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
    13621 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
    13681 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
    13741 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
    13801 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
    13861 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
    13921 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
    13981 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
    14041 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
    14101 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
    14161 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
    14221 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
    14281 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
    14341 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
    14401 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
    14461 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
    14521 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
    14581 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
    14641 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
    14701 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc
    14761 gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa cct
//
