LOCUS       sequence-121825-       13645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13645)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB421
FEATURES             Location/Qualifiers
     source          1..13645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2302..2961
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2368..2387)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3487..3506
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4720..4972
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5006..5022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5006..5022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5019..5041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5030..5046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5054..5060,5061..5078,5079..5084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5099..5120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5205..5379
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5436..6233)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5547..5566)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6157..6176
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6296..6972)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6339..6359)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7167..7191
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7712..8503
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8749..9337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9238..9257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9491..9508
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9523..9663
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9589..9608)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9749..9771
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10007..10201
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10267..11340)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11769..12398)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcttggatt tggatttgga gttgcgcttg ggattcgcct ccgccggaat ggctagtgaa
     3841 gctcgaaaaa caaagaaaaa aatcaaaggg attcagcaag ccactgcagg agtctcacaa
     3901 gacacttcgg aaaatcctaa caaaacaata gttcctgcag cattaccaca gctcacccct
     3961 accttggtgt cactgctgga ggtgattgaa cccgaggtgt tgtatgcagg atatgatagc
     4021 tctgttccag attcagcatg gagaattatg accacactca acatgttagg tgggcgtcaa
     4081 gtgattgcag cagtgaaatg ggcaaaggcg ataccaggct tcagaaactt acacctggat
     4141 gaccaaatga ccctgctaca gtactcatgg atgtttctca tggcatttgc cctgggttgg
     4201 agatcataca gacaatcaag tggaaacctg ctctgctttg ctcctgatct gattattaat
     4261 gagcagagaa tgtctctacc ctgcatgtat gaccaatgta aacacatgct gtttgtctcc
     4321 tctgaattac aaagattgca ggtatcctat gaagagtatc tctgtatgaa aaccttactg
     4381 cttctctcct cagttcctaa ggaaggtctg aagagccaag agttatttga tgagattcga
     4441 atgacttata tcaaagagct aggaaaagcc atcgtcaaaa gggaagggaa ctccagtcag
     4501 aactggcaac ggttttacca actgacaaaa cttctggact ccatgcatga ggtggttgag
     4561 aatctcctta cctactgctt ccagacattt ttggataaga ccatgagtat tgaattccca
     4621 gagatgttag ctgaaatcat cactaatcag ataccaaaat attcaaatgg aaatatcaag
     4681 aagctgctgt ttcatcaaaa atagagctcg aatttccccg atcgttcaaa catttggcaa
     4741 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     4801 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     4861 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     4921 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     4981 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5041 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5101 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt caacacattg
     5161 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     5221 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     5281 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
     5341 cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc
     5401 tagctagagg atcgatccga accccagagt cccgctcaga agaactcgtc aagaaggcga
     5461 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     5521 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     5581 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     5641 atgatattcg gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc gtcgggcatg
     5701 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     5761 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     5821 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     5881 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     5941 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     6001 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc
     6061 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     6121 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     6181 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatccc catggtcgat
     6241 cgacagatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc
     6301 gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
     6361 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
     6421 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     6481 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     6541 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     6601 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     6661 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     6721 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     6781 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
     6841 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
     6901 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
     6961 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
     7021 acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgaatt
     7081 cagaaataaa ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg
     7141 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
     7201 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
     7261 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
     7321 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
     7381 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg
     7441 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     7501 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     7561 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     7621 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     7681 gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc
     7741 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     7801 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     7861 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     7921 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     7981 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     8041 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     8101 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     8161 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     8221 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     8281 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     8341 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     8401 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     8461 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc
     8521 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca
     8581 taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata
     8641 ataagcccta cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt
     8701 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8761 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8821 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8881 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8941 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9001 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9061 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9121 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9181 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9241 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9301 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9361 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9421 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9481 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     9541 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     9601 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     9661 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     9721 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     9781 tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga
     9841 cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg
     9901 gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag
     9961 ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag
    10021 gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt
    10081 ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc
    10141 aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac
    10201 aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc
    10261 cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag
    10321 gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc
    10381 gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc
    10441 gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg
    10501 gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac
    10561 gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat
    10621 gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc
    10681 ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg
    10741 cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc
    10801 tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc
    10861 tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac
    10921 caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc
    10981 gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa
    11041 aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac
    11101 gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc
    11161 cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg
    11221 gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac
    11281 caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac
    11341 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg
    11401 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
    11461 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
    11521 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
    11581 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
    11641 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
    11701 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
    11761 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
    11821 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
    11881 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg
    11941 gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt
    12001 agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc
    12061 catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg
    12121 gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg
    12181 atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga
    12241 tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga
    12301 acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct
    12361 gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt
    12421 tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata
    12481 cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg
    12541 ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt
    12601 gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa
    12661 tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc
    12721 attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg
    12781 ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga
    12841 gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag
    12901 ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc
    12961 tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa
    13021 ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc
    13081 ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc
    13141 cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc
    13201 agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat
    13261 cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa
    13321 gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt
    13381 catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc
    13441 gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg
    13501 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
    13561 gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
    13621 tacaaatgga cgaacggata aacct
//
