LOCUS       sequence-121834-       10827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of knockdown-proof human ch-TOG tagged with
            EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
  TITLE     TACC3-ch-TOG track the growing tips of microtubules independently of
            clathrin and Aurora-A phosphorylation.
  JOURNAL   Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
  PUBMED    25596274
REFERENCE   2  (bases 1 to 10827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2015-01-16- 16"; volume: "4"; 
            issue: "2"; pages: "170-9"
COMMENT     Sequence Label: ch-TOGKDP-GFP
FEATURES             Location/Qualifiers
     source          1..10827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             complement(1425..1442)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      6767..6776
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6773..6775,6776..6778,6779..7492)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6773..6775,6776..6778,6779..7489)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6818..6839)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7079..7098)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7426..7447
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7615..7736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7652..7671)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7706..7725
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7743..8198)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7880..7901)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8092..8111
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8225..8329
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8331..8688
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8331..8522
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8354..8374)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8539..8674
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8723..9517
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8723..9517
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8777..8796)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9387..9406
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9705..9724)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9749..9796
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10125..10713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10614..10633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 aatgggagat gacagtgagt ggttgaaact gccagttgat cagaaatgtg aacacaagct
      721 gtggaaagca aggttaagtg ggtatgaaga ggccctgaag atcttccaga aaataaagga
      781 tgaaaagagc ccagagtggt ccaaattttt aggattgatc aaaaaatttg tcactgattc
      841 caatgcagtg gttcaattga aaggattaga agctgcactt gtttatgttg aaaatgccca
      901 tgtagcagga aaaaccacag gagaagttgt gtcaggtgtt gtaagtaagg tgttcaatca
      961 acctaaagct aaagccaagg agctgggcat agagatctgt cttatgtaca tagagattga
     1021 gaaaggagag gctgttcaag aagagctcct gaaaggcttg gacaataaga atcccaagat
     1081 catagtggcc tgtatagaga cactgaggaa agccttaagt gaatttggtt ccaaaatcat
     1141 cttgcttaag ccaattatca aagtgttgcc aaaactcttt gagtctcgag agaaggctgt
     1201 tcgagatgaa gccaaactaa ttgctgtgga gatttacaga tggattcggg atgctctgag
     1261 acccccatta caaaatataa actctgttca gttgaaagaa ctagaagaag aatgggtcaa
     1321 actgccaaca agtgctccta gacctactcg atttcttcgt tcccaacaag aactagaagc
     1381 taaattggaa caacaacagt ctgctggtgg agatgctgaa ggaggtggtg atgatggtga
     1441 tgaggtgcca caaatagatg cttatgagct tttagaagct gtagaaatcc tttccaaact
     1501 tcccaaagac ttttatgaca aaattgaggc aaaaaaatgg caagagagaa aagaggccct
     1561 ggagtctgta gaagtactaa taaaaaaccc caaactggaa gctggcgatt atgcagattt
     1621 agtaaaagca ttaaagaagg ttgttggaaa ggacaccaat gtcatgttgg tggctttggc
     1681 agcaaaatgt cttactggcc tggctgttgg gctaaggaag aaatttggac aatatgcagg
     1741 acatgttgtg ccaaccatct tggagaaatt caaagagaag aaacctcaag tggtacaagc
     1801 cctgcaggag gcaattgatg caatcttcct tactaccaca ctacagaaca tcagtgagga
     1861 tgttttagca gtaatggata ataaaaatcc aaccatcaag cagcagacat ctctttttat
     1921 tgcaagaagt ttccgccact gcactgcttc taccctgcca aagagcttgc taaagccctt
     1981 ttgtgctgca ctacttaagc acatcaatga ttctgctcct gaagtcagag atgccgcatt
     2041 tgaagcattg ggtactgctt tgaaggtggt tggcgagaaa gcagtaaacc cattcctagc
     2101 tgatgtggac aaactcaagc ttgataagat caaagaatgt tcagaaaagg tagaactgat
     2161 acatggtaag aaagctggac tagctgctga taagaaggaa ttcaaacctc tgcctggaag
     2221 gactgctgct tcaggggctg caggagataa ggacacaaag gacatttctg cacccaaacc
     2281 aggacctcta aaaaaggcac ctgctgctaa ggctggtggg ccaccaaaaa aggggaaacc
     2341 agctgcacca ggaggcgcag ggaatactgg aaccaagaac aagaaaggac tggagactaa
     2401 agaaatagtg gagcctgagc tctcgataga agtatgtgaa gaaaaagctt cagctgttct
     2461 tccccctacc tgtatacagc ttcttgacag cagtaactgg aaagaaaggc tggcttgtat
     2521 ggaagagttc cagaaggctg ttgagctaat ggaccgaact gaaatgccat gccaggcatt
     2581 agtgaggatg ctagccaaga aacctggatg gaaagaaact aattttcagg tgatgcaaat
     2641 gaagcttcat atagttgctt tgattgccca gaagggaaat ttttccaaaa cgtcagctca
     2701 ggttgtatta gatggccttg tggacaagat tggagatgtg aaatgtggga acaatgcaaa
     2761 agaagctatg acagcaatag ccgaagcctg tatgttacca tggactgctg aacaggttgt
     2821 gtcaatggct ttctcacaaa agaatcccaa aaatcagtca gaaactctga attggctatc
     2881 aaatgccata aaagaatttg gtttttctgg gttgaatgtc aaagctttca ttagcaatgt
     2941 gaagacagct cttgctgcaa caaacccagc tgtgaggact gctgccataa ccctgcttgg
     3001 cgtgatgtat ctgtatgttg gtccctcttt gcgaatgttc tttgaggatg agaagcctgc
     3061 cctcctatcc cagatagatg cagaatttga gaagatgcag ggacaaagtc cacctgctcc
     3121 aaccagagga atttccaagc atagcacaag tggtacagat gaaggagaag atggagatga
     3181 accagatgac gggagcaatg atgtcgttga tcttttgccg aggacggaga tcagtgataa
     3241 aatcacttca gagttggtat ctaagattgg tgataagaat tggaagatta ggaaagaagg
     3301 cctagatgaa gtggcaggta ttattaatga cgcaaaattt atccaaccga atataggtga
     3361 acttccaact gccttgaagg gtcgactcaa tgattcaaat aaaatcttgg tacagcaaac
     3421 gctgaatatc ctgcaacaac tggcagtagc catgggccca aatattaagc aacatgtaaa
     3481 aaatttaggc atccctatca tcacagtcct tggagacagc aagaacaatg ttcgagctgc
     3541 tgccctagcg actgtgaatg cttgggcaga acagactggc atgaaggaat ggctggaagg
     3601 agaagatctt tctgaagagc tcaaaaagga aaatcctttc ttgaggcaag agcttctggg
     3661 ctggctggct gagaaactac ctactcttcg ttccacccct acagacctta tcctttgtgt
     3721 tcctcatctc tactcctgcc tagaagatcg aaatggagat gtgcgaaaga aggcccaaga
     3781 tgccttgcca ttcttcatga tgcatttagg atatgaaaaa atggccaagg ctactgggaa
     3841 actaaagcca acttctaaag atcaggtatt ggccatgcta gagaaagcca aagttaacat
     3901 gccagccaag cctgctccac ccactaaagc aacttctaaa ccaatgggag ggtccgctcc
     3961 agccaaattc cagcctgcat cagcacctgc tgaagattgt atttccagca gtacagaacc
     4021 caaacctgat ccaaaaaagg ccaaagctcc aggattatcc tctaaagcaa agagtgcaca
     4081 agggaagaag atgccaagca aaaccagctt aaaggaggat gaagacaaat ccgggcctat
     4141 ttttattgtt gttccaaatg gaaaagagca aaggatgaaa gatgaaaaag gattgaaggt
     4201 gctaaagtgg aattttacta ccccacggga tgaatacatt gagcaactaa agactcaaat
     4261 gtctagctgt gtggctaaat ggttacaaga tgagatgttt cactcagact ttcagcatca
     4321 taacaaagcc cttgctgtta tggttgatca cttggagagt gaaaaagaag gagttattgg
     4381 ttgcctggat cttatcttaa agtggcttac cctgaggttt tttgacacca atacaagcgt
     4441 cctgatgaaa gcactagaat atttaaaatt gctcttcacc ttgctaagtg aagaagaata
     4501 tcatcttact gagaatgaag catcttcctt catcccctat cttgtcgtca aggttggaga
     4561 accaaaggat gtcattcgta aagatgttcg tgccatcctg aaccggatgt gccttgtcta
     4621 cccagctagc aagatgtttc cctttatcat ggaaggaacc aaatccaaaa actctaagca
     4681 gagagcagag tgcctggaag agctgggatg tctggttgag tcctatggca tgaatgtttg
     4741 ccaaccaacc ccaggaaaag ccttaaagga aatagctgtt cacataggag accgtgacaa
     4801 tgctgtacgc aatgctgcac tcaacaccat tgtaacggtg tacaatgtac atggggatca
     4861 ggtgttcaaa ctgattggaa atctttctga aaaggatatg agcatgctcg aggagaggat
     4921 taagcggtca gcaaagagac cctctgctgc accaataaaa caggtggaag agaaacctca
     4981 gcgtgcacag aacataagct ccaatgccaa catgttacgc aagggaccag ctgaggacat
     5041 gtcttccaaa ctcaaccaag cccgaagcat gagtgggcat cctgaggcag cccagatggt
     5101 ccgccgagaa ttccagctgg atctagatga gattgagaat gacaatggta cagtccgatg
     5161 tgaaatgcca gaacttgttc agcacaaact ggatgacatt tttgagccag tccttattcc
     5221 tgaacccaag atccgggctg tttctccaca cttcgatgac atgcacagta atacagcatc
     5281 cacaatcaat ttcattatct cccaagtagc cagtggtgac atcaacacaa gtatccaagc
     5341 tctgacacag atcgatgagg tcctgagaca ggaagacaaa gctgaagcca tgtccggcca
     5401 tattgatcag tttctgatag ccacttttat gcagctaaga ctcatctaca acacacacat
     5461 ggcagatgag aaattggaga aggacgagat catcaagttg tatagctgta tcattggcaa
     5521 catgatttcg ctgtttcaga tagagagcct tgcccgggag gcctccactg gagtactaaa
     5581 agacctaatg catggcctca tcaccttaat gctggattct cggattgaag atcttgagga
     5641 aggacaacag gtcatccgct ctgtgaacct cttggtggtg aaggttctgg agaagtcaga
     5701 ccagaccaac atcctgagtg ccctacttgt tttgctccaa gacagcctgc tagcaacagc
     5761 cagttctccc aaattctcag agcttgttat gaagtgtctc tggagaatgg ttcgactgtt
     5821 gcctgatacc atcaatagca ttaacctaga cagaattctt ctggatatcc acattttcat
     5881 gaaggtcttc cccaaagaga aactgaagca atgcaaaagt gaatttccca taaggaccct
     5941 aaagaccctg ctacacacct tatgcaaatt aaaagggccc aagatcctgg accacctaac
     6001 gatgatcgac aacaaaaacg agtctgagct ggaggcccat ctctgccgga tgatgaagca
     6061 cagtatggac cagactggga gcaagtctga taaggaaaca gaaaagggag catctcgaat
     6121 agatgaaaaa tcatcaaagg ccaaagtgaa tgatttctta gctgagattt ttaagaagat
     6181 tggctctaaa gaaaacacta aagagggact agcagagtta tatgaatata agaagaaata
     6241 ctcagatgct gacattgaac catttctgaa aaattcctca cagttcttcc agagctatgt
     6301 cgaaagaggc cttcgggtga ttgagatgga gagggagggc aaaggtcgta tttccacttc
     6361 aacaggcatc tcccctcaga tggaagtcac atgtgtgccc acgcccacaa gcacagtgtc
     6421 ctccataggt aacacaaatg gggaagaagt ggggccatct gtctacttgg aaaggctaaa
     6481 gatcctccga cagcgatgtg gtctggacaa cacaaagcaa gatgaccgac ctcctttgac
     6541 ctctttgctc tccaaaccag cagttcctac tgtcgcctct tccacagaca tgctacattc
     6601 caagttgtct cagctccggg agtcacggga gcagcaccag cattcagacc tggattctaa
     6661 ccagactcac tcttcaggaa ctgtgacctc ctcctcctcc acagctaaca tagacgactt
     6721 gaaaaaaaga ctggagagaa taaagagcag tcgcaaagga tccatcgcca ccatggtgag
     6781 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     6841 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     6901 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     6961 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     7021 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     7081 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     7141 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     7201 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     7261 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     7321 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     7381 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     7441 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     7501 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     7561 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     7621 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7681 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     7741 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     7801 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     7861 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     7921 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     7981 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     8041 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     8101 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     8161 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     8221 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8281 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     8341 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     8401 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8461 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     8521 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8581 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8641 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     8701 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     8761 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     8821 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     8881 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     8941 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     9001 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     9061 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     9121 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     9181 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     9241 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     9301 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     9361 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     9421 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     9481 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     9541 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     9601 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     9661 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     9721 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     9781 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     9841 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     9901 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     9961 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10021 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10081 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10141 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10201 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10261 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10321 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10381 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10441 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10501 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10561 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10621 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10681 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10741 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10801 gtggataacc gtattaccgc catgcat
//
