LOCUS       sequence-121841-       11543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged
            with EGFP along with shRNA to knock down endogenous ch-TOG in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
  TITLE     TACC3-ch-TOG track the growing tips of microtubules independently of
            clathrin and Aurora-A phosphorylation.
  JOURNAL   Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
  PUBMED    25596274
REFERENCE   2  (bases 1 to 11543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2015-01-16- 16"; volume: "4"; 
            issue: "2"; pages: "170-9"
COMMENT     Sequence Label: pBrain-ch-TOGKDP-GFP-shch-TOG
FEATURES             Location/Qualifiers
     source          1..11543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..86
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     78..95
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     79..95
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     102..121
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        102..120
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     153..169
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        189..403
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     324..343
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(476..492)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(477..493)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(521..539)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(560..576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(560..576)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(573..595)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    584..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(608..614,615..632,633..638))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    653..674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     enhancer        777..1080
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1081..1284
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1235..1255
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             complement(2141..2158)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      7483..7492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7489..7491,7492..7494,7495..8208)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7489..7491,7492..7494,7495..8205)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7534..7555)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7795..7814)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8142..8163
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8331..8452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8368..8387)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8422..8441
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8459..8914)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8596..8617)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8808..8827
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8941..9045
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9047..9404
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9047..9238
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9070..9090)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9255..9390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9439..10233
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9439..10233
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9493..9512)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10103..10122
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10421..10440)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10465..10512
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10841..11429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11330..11349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
       61 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
      121 gcgaattgga gctccaccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc
      181 aggaattcga acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta
      241 ggcgggaaca cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag
      301 tggcgccctg caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa
      361 tgtctttgga tttgggaatc ttataagttc tgtatgagac cacagatccc ctgctccaca
      421 gcaaactctc ttcaagagag agagtttgct gtggagcatt tttggaaaag cttatcgata
      481 ccgtcgacct cgaggggggg cccggtacca gcttttgttc cctttagtga gggttaattt
      541 cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      601 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      661 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      721 gccagatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      781 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      841 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      901 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      961 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     1021 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     1081 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     1141 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     1201 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     1261 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
     1321 actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccggaatg
     1381 ggagatgaca gtgagtggtt gaaactgcca gttgatcaga aatgtgaaca caagctgtgg
     1441 aaagcaaggt taagtgggta tgaagaggcc ctgaagatct tccagaaaat aaaggatgaa
     1501 aagagcccag agtggtccaa atttttagga ttgatcaaaa aatttgtcac tgattccaat
     1561 gcagtggttc aattgaaagg attagaagct gcacttgttt atgttgaaaa tgcccatgta
     1621 gcaggaaaaa ccacaggaga agttgtgtca ggtgttgtaa gtaaggtgtt caatcaacct
     1681 aaagctaaag ccaaggagct gggcatagag atctgtctta tgtacataga gattgagaaa
     1741 ggagaggctg ttcaagaaga gctcctgaaa ggcttggaca ataagaatcc caagatcata
     1801 gtggcctgta tagagacact gaggaaagcc ttaagtgaat ttggttccaa aatcatcttg
     1861 cttaagccaa ttatcaaagt gttgccaaaa ctctttgagt ctcgagagaa ggctgttcga
     1921 gatgaagcca aactaattgc tgtggagatt tacagatgga ttcgggatgc tctgagaccc
     1981 ccattacaaa atataaactc tgttcagttg aaagaactag aagaagaatg ggtcaaactg
     2041 ccaacaagtg ctcctagacc tactcgattt cttcgttccc aacaagaact agaagctaaa
     2101 ttggaacaac aacagtctgc tggtggagat gctgaaggag gtggtgatga tggtgatgag
     2161 gtgccacaaa tagatgctta tgagctttta gaagctgtag aaatcctttc caaacttccc
     2221 aaagactttt atgacaaaat tgaggcaaaa aaatggcaag agagaaaaga ggccctggag
     2281 tctgtagaag tactaataaa aaaccccaaa ctggaagctg gcgattatgc agatttagta
     2341 aaagcattaa agaaggttgt tggaaaggac accaatgtca tgttggtggc tttggcagca
     2401 aaatgtctta ctggcctggc tgttgggcta aggaagaaat ttggacaata tgcaggacat
     2461 gttgtgccaa ccatcttgga gaaattcaaa gagaagaaac ctcaagtggt acaagccctg
     2521 caggaggcaa ttgatgcaat cttccttact accacactac agaacatcag tgaggatgtt
     2581 ttagcagtaa tggataataa aaatccaacc atcaagcagc agacatctct ttttattgca
     2641 agaagtttcc gccactgcac tgcttctacc ctgccaaaga gcttgctaaa gcccttttgt
     2701 gctgcactac ttaagcacat caatgattct gctcctgaag tcagagatgc cgcatttgaa
     2761 gcattgggta ctgctttgaa ggtggttggc gagaaagcag taaacccatt cctagctgat
     2821 gtggacaaac tcaagcttga taagatcaaa gaatgttcag aaaaggtaga actgatacat
     2881 ggtaagaaag ctggactagc tgctgataag aaggaattca aacctctgcc tggaaggact
     2941 gctgcttcag gggctgcagg agataaggac acaaaggaca tttctgcacc caaaccagga
     3001 cctctaaaaa aggcacctgc tgctaaggct ggtgggccac caaaaaaggg gaaaccagct
     3061 gcaccaggag gcgcagggaa tactggaacc aagaacaaga aaggactgga gactaaagaa
     3121 atagtggagc ctgagctctc gatagaagta tgtgaagaaa aagcttcagc tgttcttccc
     3181 cctacctgta tacagcttct tgacagcagt aactggaaag aaaggctggc ttgtatggaa
     3241 gagttccaga aggctgttga gctaatggac cgaactgaaa tgccatgcca ggcattagtg
     3301 aggatgctag ccaagaaacc tggatggaaa gaaactaatt ttcaggtgat gcaaatgaag
     3361 cttcatatag ttgctttgat tgcccagaag ggaaattttt ccaaaacgtc agctcaggtt
     3421 gtattagatg gccttgtgga caagattgga gatgtgaaat gtgggaacaa tgcaaaagaa
     3481 gctatgacag caatagccga agcctgtatg ttaccatgga ctgctgaaca ggttgtgtca
     3541 atggctttct cacaaaagaa tcccaaaaat cagtcagaaa ctctgaattg gctatcaaat
     3601 gccataaaag aatttggttt ttctgggttg aatgtcaaag ctttcattag caatgtgaag
     3661 acagctcttg ctgcaacaaa cccagctgtg aggactgctg ccataaccct gcttggcgtg
     3721 atgtatctgt atgttggtcc ctctttgcga atgttctttg aggatgagaa gcctgccctc
     3781 ctatcccaga tagatgcaga atttgagaag atgcagggac aaagtccacc tgctccaacc
     3841 agaggaattt ccaagcatag cacaagtggt acagatgaag gagaagatgg agatgaacca
     3901 gatgacggga gcaatgatgt cgttgatctt ttgccgagga cggagatcag tgataaaatc
     3961 acttcagagt tggtatctaa gattggtgat aagaattgga agattaggaa agaaggccta
     4021 gatgaagtgg caggtattat taatgacgca aaatttatcc aaccgaatat aggtgaactt
     4081 ccaactgcct tgaagggtcg actcaatgat tcaaataaaa tcttggtaca gcaaacgctg
     4141 aatatcctgc aacaactggc agtagccatg ggcccaaata ttaagcaaca tgtaaaaaat
     4201 ttaggcatcc ctatcatcac agtccttgga gacagcaaga acaatgttcg agctgctgcc
     4261 ctagcgactg tgaatgcttg ggcagaacag actggcatga aggaatggct ggaaggagaa
     4321 gatctttctg aagagctcaa aaaggaaaat cctttcttga ggcaagagct tctgggctgg
     4381 ctggctgaga aactacctac tcttcgttcc acccctacag accttatcct ttgtgttcct
     4441 catctctact cctgcctaga agatcgaaat ggagatgtgc gaaagaaggc ccaagatgcc
     4501 ttgccattct tcatgatgca tttaggatat gaaaaaatgg ccaaggctac tgggaaacta
     4561 aagccaactt ctaaagatca ggtattggcc atgctagaga aagccaaagt taacatgcca
     4621 gccaagcctg ctccacccac taaagcaact tctaaaccaa tgggagggtc cgctccagcc
     4681 aaattccagc ctgcatcagc acctgctgaa gattgtattt ccagcagtac agaacccaaa
     4741 cctgatccaa aaaaggccaa agctccagga ttatcctcta aagcaaagag tgcacaaggg
     4801 aagaagatgc caagcaaaac cagcttaaag gaggatgaag acaaatccgg gcctattttt
     4861 attgttgttc caaatggaaa agagcaaagg atgaaagatg aaaaaggatt gaaggtgcta
     4921 aagtggaatt ttactacccc acgggatgaa tacattgagc aactaaagac tcaaatgtct
     4981 agctgtgtgg ctaaatggtt acaagatgag atgtttcact cagactttca gcatcataac
     5041 aaagcccttg ctgttatggt tgatcacttg gagagtgaaa aagaaggagt tattggttgc
     5101 ctggatctta tcttaaagtg gcttaccctg aggttttttg acaccaatac aagcgtcctg
     5161 atgaaagcac tagaatattt aaaattgctc ttcaccttgc taagtgaaga agaatatcat
     5221 cttactgaga atgaagcatc ttccttcatc ccctatcttg tcgtcaaggt tggagaacca
     5281 aaggatgtca ttcgtaaaga tgttcgtgcc atcctgaacc ggatgtgcct tgtctaccca
     5341 gctagcaaga tgtttccctt tatcatggaa ggaaccaaat ccaaaaactc taagcagaga
     5401 gcagagtgcc tggaagagct gggatgtctg gttgagtcct atggcatgaa tgtttgccaa
     5461 ccaaccccag gaaaagcctt aaaggaaata gctgttcaca taggagaccg tgacaatgct
     5521 gtacgcaatg ctgcactcaa caccattgta acggtgtaca atgtacatgg ggatcaggtg
     5581 ttcaaactga ttggaaatct ttctgaaaag gatatgagca tgctcgagga gaggattaag
     5641 cggtcagcaa agagaccctc tgctgcacca ataaaacagg tggaagagaa acctcagcgt
     5701 gcacagaaca taagctccaa tgccaacatg ttacgcaagg gaccagctga ggacatgtct
     5761 tccaaactca accaagcccg aagcatgagt gggcatcctg aggcagccca gatggtccgc
     5821 cgagaattcc agctggatct agatgagatt gagaatgaca atggtacagt ccgatgtgaa
     5881 atgccagaac ttgttcagca caaactggat gacatttttg agccagtcct tattcctgaa
     5941 cccaagatcc gggctgtttc tccacacttc gatgacatgc acagtaatac agcatccaca
     6001 atcaatttca ttatctccca agtagccagt ggtgacatca acacaagtat ccaagctctg
     6061 acacagatcg atgaggtcct gagacaggaa gacaaagctg aagccatgtc cggccatatt
     6121 gatcagtttc tgatagccac ttttatgcag ctaagactca tctacaacac acacatggca
     6181 gatgagaaat tggagaagga cgagatcatc aagttgtata gctgtatcat tggcaacatg
     6241 atttcgctgt ttcagataga gagccttgcc cgggaggcct ccactggagt actaaaagac
     6301 ctaatgcatg gcctcatcac cttaatgctg gattctcgga ttgaagatct tgaggaagga
     6361 caacaggtca tccgctctgt gaacctcttg gtggtgaagg ttctggagaa gtcagaccag
     6421 accaacatcc tgagtgccct acttgttttg ctccaagaca gcctgctagc aacagccagt
     6481 tctcccaaat tctcagagct tgttatgaag tgtctctgga gaatggttcg actgttgcct
     6541 gataccatca atagcattaa cctagacaga attcttctgg atatccacat tttcatgaag
     6601 gtcttcccca aagagaaact gaagcaatgc aaaagtgaat ttcccataag gaccctaaag
     6661 accctgctac acaccttatg caaattaaaa gggcccaaga tcctggacca cctaacgatg
     6721 atcgacaaca aaaacgagtc tgagctggag gcccatctct gccggatgat gaagcacagt
     6781 atggaccaga ctgggagcaa gtctgataag gaaacagaaa agggagcatc tcgaatagat
     6841 gaaaaatcat caaaggccaa agtgaatgat ttcttagctg agatttttaa gaagattggc
     6901 tctaaagaaa acactaaaga gggactagca gagttatatg aatataagaa gaaatactca
     6961 gatgctgaca ttgaaccatt tctgaaaaat tcctcacagt tcttccagag ctatgtcgaa
     7021 agaggccttc gggtgattga gatggagagg gagggcaaag gtcgtatttc cacttcaaca
     7081 ggcatctccc ctcagatgga agtcacatgt gtgcccacgc ccacaagcac agtgtcctcc
     7141 ataggtaaca caaatgggga agaagtgggg ccatctgtct acttggaaag gctaaagatc
     7201 ctccgacagc gatgtggtct ggacaacaca aagcaagatg accgacctcc tttgacctct
     7261 ttgctctcca aaccagcagt tcctactgtc gcctcttcca cagacatgct acattccaag
     7321 ttgtctcagc tccgggagtc acgggagcag caccagcatt cagacctgga ttctaaccag
     7381 actcactctt caggaactgt gacctcctcc tcctccacag ctaacataga cgacttgaaa
     7441 aaaagactgg agagaataaa gagcagtcgc aaaggatcca tcgccaccat ggtgagcaag
     7501 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     7561 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     7621 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     7681 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     7741 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     7801 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     7861 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     7921 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     7981 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     8041 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     8101 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     8161 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
     8221 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     8281 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     8341 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8401 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa
     8461 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     8521 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     8581 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8641 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8701 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8761 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     8821 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     8881 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     8941 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9001 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     9061 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     9121 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     9181 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     9241 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9301 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9361 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     9421 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     9481 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     9541 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     9601 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     9661 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     9721 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     9781 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     9841 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     9901 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     9961 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
    10021 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
    10081 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
    10141 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
    10201 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
    10261 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
    10321 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    10381 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
    10441 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
    10501 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
    10561 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
    10621 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
    10681 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10981 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    11041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    11101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    11161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    11221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    11281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    11341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    11401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    11461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    11521 ataaccgtat taccgccatg cat
//
